Recombinant Rat B7-H2 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 10419-B7
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived rat B7-H2 protein
Rat B7-H2 (Glu25-Lys261) Accession # XP_006256322.1 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Glu25
Predicted Molecular Mass
54 kDa
SDS-PAGE
70-90 kDa, under reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Rat B7-H2 Fc Chimera (Catalog # 10419-B7) is Immobilized at 1.0 μg/mL (100 μL/well), the concentration of Recombinant Mouse ICOS Fc Chimera (Catalog # 168-CS) that produces 50% optimal binding response is found to be approximately 10-100 ng/mL.
When Recombinant Rat B7-H2 Fc Chimera (Catalog # 10419-B7) is Immobilized at 1.0 μg/mL (100 μL/well), the concentration of Recombinant Mouse ICOS Fc Chimera (Catalog # 168-CS) that produces 50% optimal binding response is found to be approximately 10-100 ng/mL.
Scientific Data Images for Recombinant Rat B7-H2 Fc Chimera Protein, CF
Recombinant Rat B7-H2 Fc Chimera Protein Binding Activity
When Recombinant Rat B7-H2 Fc Chimera (Catalog # 10419-B7) is immobilized at 1.0 µg/mL (100 µL/well), the concentration of Recombinant Mouse ICOS Fc Chimera (Catalog # 168-CS) that produces 50% the optimal binding response is found to be approximately 10-100 ng/mL.Recombinant Rat B7-H2 Fc Chimera Protein SDS-PAGE
2 μg/lane of Recombinant Rat B7-H2 Fc Chimera (Catalog # 10419-B7) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-90 kDa and 140-180 kDa, respectively.Formulation, Preparation and Storage
10419-B7
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 200 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: B7-H2
References
- Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
- Wang, S. et al. (2000) Blood 96:2808.
- Yoshinaga, S.K. et al. (2000) Int. Immunol. 12:1439.
- Yoshinaga, S.K. et al. (1999) Nature 402:827.
- Faget, J. et al. (2012) Cancer Res. 72:6130.
- Yao, S. et al. (2011) Immunity 32:729.
- Watanabe, M. et al. (2008) J. Immunol. 180:5222.
- Wong, S.-C. et al. (2003) Blood 102:1381.
- Hu, Y.-L. et al. (2009) J. Immunol. 182:1421.
- Kadkhoda, K. et al. (2010) J. Immunol. 184:3780.
- Kadkhoda, K. et al. (2011) Int. Immunol. 23:239.
- Gajewska, B.U. et al. (2005) J. Immunol. 174:3000.
- Akbari, O. et al. (2002) Nat. Med. 8:1024.
- Her, M. et al. (2009) Lupus 18:501.
- Wang, F. et al. (2012) J. Clin. Immunol. 32:1253.
- Maazi H. et al. (2015) Immunity. 42:538.
Long Name
B7 Homolog 2
Alternate Names
B7H2, B7RP-1, CD275, GL50, ICOSL, ICOSLG
Gene Symbol
ICOSLG
UniProt
Additional B7-H2 Products
Product Documents for Recombinant Rat B7-H2 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Rat B7-H2 Fc Chimera Protein, CF
For research use only
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