Recombinant Rat CLEC10A/CD301 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9037-CL

R&D Systems, part of Bio-Techne
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9037-CL-050
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Key Product Details

Source

CHO

Accession #

NP_071788

Conjugate

Unconjugated

Applications

Bioactivity

View all CLEC10A/CD301 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived rat CLEC10A/CD301 protein
Gln59-Ser306, with an N-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

29 kDa

SDS-PAGE

34-42 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Rat CLEC10A/CD301 is immobilized at 2.5 µg/mL (100 µL/well), the concentration of biotinylated Recombinant Viral EBOV GP that produces 50% of the optimal binding response is approximately 1-6 μg/mL

Formulation, Preparation and Storage

9037-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC10A/CD301

CLEC10A, also known as macrophage galactose/N-acetyl-galactosamine (GalNAc) specific lectin (MGL), CD301, DC-ASGPR, and HML, is a 40 kDa type II transmembrane glycoprotein in the C-type lectin family (1). Human and rat carry a single gene for CLEC10A/MGL, while mouse has two closely related MGL1 and MGL2 genes. Rat CLEC10A consists of a 37 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment, and a 248 aa extracellular domain (ECD) with one carbohydrate recognition domain and a neck region (2). Within the ECD, rat CLEC10A shares 56% and 79% aa sequence identity with human CLEC10A and mouse CLEC10A/MGL1, respectively. CLEC10A is expressed on immature myleloid dendritic cells, Langerhans cells, and alternatively activated (tolerogenic) macrophages and is upregulated by the immunosuppressant dexamethasone (3-8). It is up-regulated on pro-inflammatory monocytes in obesity and promotes their trafficking to adipose tissue (9). CLEC10A selectively binds and internalizes terminal nonsialylated alpha- or beta-linked GalNAc moieties on O-linked carbohydrates including the Tn carcinoma antigen (3, 4, 10-12). Similar ligand preference is exhibited by mouse MGL2 but not MGL1 (13). CLEC10A expressed on tolerogenic dendritic cells binds carbohydrate determinants on CD45 (RA, RB, and RC but not RO isoforms) on T, NK, and B cells (6). This interaction inhibits effector T cell activation and induces their apoptosis (6). CLEC10A also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity (14).

References

  1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
  2. Ii, M. et al. (1990) J. Biol. Chem. 265:11295.
  3. Higashi, N. et al. (2002) J. Biol. Chem. 277:20686.
  4. Valladeau, J. et al. (2001) J. Immunol. 167:5767.
  5. van Vliet, S.J. et al. (2006) Immunobiology 211:577.
  6. van Vliet, S.J. et al. (2006) Nat. Immunol. 7:1200.
  7. Raes, G. et al. (2005) J. Leukoc. Biol. 77:321.
  8. Dupasquier, M. et al. (2006) J. Leukoc. Biol. 80:838.
  9. Westcott, D.J. et al. (2009) J. Exp. Med. 206:3143.
  10. Suzuki, N. et al. (1996) J. Immunol. 156:128.
  11. van Vliet, S.J. et al. (2005) Int. Immunol. 17:661.
  12. Higashi, N. et al. (2002) Int. Immunol. 14:545.
  13. Tsuiji, M. et al. (2002) J. Biol. Chem. 277:28892.
  14. Takada, A. et al. (2004) J. Virol. 78:2943.

Long Name

Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 1

Alternate Names

CD301, CLECSF14, HML2, MGL

Entrez Gene IDs

10462 (Human)

Gene Symbol

CLEC10A

UniProt

NP_071788

Additional CLEC10A/CD301 Products

Product Documents for Recombinant Rat CLEC10A/CD301 Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Rat CLEC10A/CD301 Protein, CF

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