Skip to main content

Recombinant Human Ribonuclease A His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12164

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP3-12164-100ug
NBP3-12164-10ug
NBP3-12164-20ug
NBP3-12164-250ug
NBP3-12164-500ug
NBP3-12164-50ug

Key Product Details

Source

HEK293

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

Product Specifications

Description

A recombinant protein with a His tag corresponding to the amino acids 29-156 of Human Ribonuclease A

Source: HEK293

Amino Acid Sequence: KESRAKKFQR QHMDSDSSPS SSSTYCNQMM RRRNMTQGRC KPVNTFVHEP LVDVQNVCFQ EKVTCKNGQG NCYKSNSSMH ITDCRLTNGS RYPNCAYRTS PKERHIIVAC EGSPYVPVHF DASVEDST

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1 EU per 1ug of protein (determined by LAL method)

Predicted Molecular Mass

15.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 5 X 10^4 unit/mg, and is defined as the amount of enzyme that cleaves 1.0 pmole of RNase probe per minute at 25C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Ribonuclease A His Protein

SDS-PAGE: Recombinant Human Ribonuclease A His Protein [NBP3-12164]

SDS-PAGE: Recombinant Human Ribonuclease A His Protein [NBP3-12164]

SDS-Page: Recombinant Human Ribonuclease A His Protein [NBP3-12164] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation and Storage

NBP3-12164
Formulation PBS (pH 7.4), 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ribonuclease A

This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq]

Alternate Names

EC 3.1.27.5, HP-RNase, MGC12408, RIB1, RIB-1, Ribonuclease 1, Ribonuclease A, ribonuclease pancreatic, ribonuclease, RNase A family, 1 (pancreatic), RNase A, RNase UpI-1, RNS1RNase 1

Gene Symbol

RNASE1

Additional Ribonuclease A Products

Product Documents for Recombinant Human Ribonuclease A His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human Ribonuclease A His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...