Recombinant Human TIMP-2 Protein

Name: Recombinant Human TIMP-2 Protein
Brand: Novus Biologicals
SKU: NBP2-61356

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61356

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Product Specifications
Preparation & Storage
Background
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Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity

View all TIMP-2 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 194 amino acids corresponding to TIMP-2 Source: E. coli

Uniprot ID: P16035

Amino Acid Sequence: CSCSPVHPQQ AFCNADVVIR AKAVSEKEVD SGNDIYGNPI KRIQYEIKQI KMFKGPEKDI EFIYTAPSSA VCGVSLDVGG KKEYLIAGKA EGDGKMHITL CDFIVPWDTL STTQKKSLNH RYQMGCECKI TRCPMIPCYI SSPDECLWMD WVTEKNINGH QAKFFACIKR SDGSCAWYRG AAPPKQEFLD IEDP

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of TIMP-2 as determined by LAL method.

Predicted Molecular Mass

21.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The biologically active as determined by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-61356

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 3% Trehalose.
Preservative	Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: TIMP-2

The TIMP2 gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role a

Long Name

Tissue Inhibitors of Metalloproteinases 2

Alternate Names

TIMP2

Gene Symbol