Ubiquitin-Propargylamide Protein
R&D Systems, part of Bio-Techne | Catalog # U-214
Key Product Details
Source
Synthetic
Accession #
Structure / Form
Modified
Conjugate
Propargylamide
Applications
Bioactivity
Product Specifications
Source
Chemically Synthesized Ubiquitin protein
Met1 - Gly75
Met1 - Gly75
Purity
>95%, by HPLC.
Predicted Molecular Mass
8.5 kDa
Activity
Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin-Propargylamide concentration of 0.5-5 μM.
Reviewed Applications
Read 1 review rated 4 using U-214 in the following applications:
Formulation, Preparation and Storage
U-214
| Formulation | Lyophilized from a solution containing Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 2 mg/ml in DMSO. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Ubiquitin
Ubiquitin-Propargylamine (Ub-PA) is a potent, irreversible and specific inhibitor of C-terminal hydrolases (UCHs), ubiquitin-specific proteases (USPs), and other deubiquitinases including the Otubains (OTU’s). When mixed together, the reactive site cysteine of deubiquitinases and the C-terminal alkyne functional group of Ub-PA rapidly form a covalent bond (vinyl thioether) that is resistant to denaturing conditions. This product is useful for inhibiting the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation.
References
- Ekkebus, R., et al. (2013) J. Am. Chem. Soc 135:2867.
- Sommer, S., et al. (2013) Bioorg Med Chem. 21:2511.
Alternate Names
UBB
Gene Symbol
UBB
UniProt
Additional Ubiquitin Products
Product Documents for Ubiquitin-Propargylamide Protein
Product Specific Notices for Ubiquitin-Propargylamide Protein
For research use only
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