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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse IgM heavy and light chains

Specificity

Mouse IgM specific IgG was purified by goat IgG affinity chromatography and then adsorbed to eliminate human cross reactivity. The IgG fraction was digested with pepsin to generate F(ab')2 fragments which have a reduced ability to interact with Fc receptors expressed on a variety of cells. 

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Goat F(ab')2 Anti-mouse IgM-Fluorescein Antibody

Application
Recommended Usage

Flow Cytometry

Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter molecule, is of mouse origin and is of the IgM class.The recommended concentration is 10 µL/106 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgM

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin M

Alternate Names

AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH

Additional IgM Products

Product Documents for Goat F(ab')2 Anti-mouse IgM-Fluorescein Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Goat F(ab')2 Anti-mouse IgM-Fluorescein Antibody

For research use only

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