Goat anti-Canine IgE Heavy Chain Secondary Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB7343
![ELISA: Goat anti-Canine IgE Heavy Chain Secondary Antibody [NB7343]](https://resources.bio-techne.com/images/products/Goat-anti-Canine-IgE-Secondary-Antibody-ELISA-NB7343-img0001.jpg "ELISA: Goat anti-Canine IgE Heavy Chain Secondary Antibody [NB7343]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Canine
Applications
Dot Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Dog IgE
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with dog IgE. This may cross react with IgE from other species.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Goat anti-Canine IgE Heavy Chain Secondary Antibody
ELISA: Goat anti-Canine IgE Heavy Chain Secondary Antibody [NB7343]
ELISA: Goat anti-Canine IgE Heavy Chain Secondary Antibody [NB7343] - Sandwich ELISA for total dog IgE measurement showing standard curve at 2 different coating antibody concentrations. Coating: 1ug/ml in carbonate buffer, pH 9.6. Standard: purified dog IgE, range: 156pg/ml to 15.000 pg/ml. Detection with secondary antibody (HRP-labeled) and TMB substrate. This image was submitted via customer Review.
Dot Blot: Goat anti-Canine IgE Secondary Antibody [NB7343] - D.p. extracts have effects on MCs though the IgE-FcepsilonRI axis. SDS-PAGE of recombinants (300 ng) and Western blotting (168.8 ng, for rDer p 15; 143.8 ng of rDer p 18 per gel lane) of specific IgE in canine sera. Image collected and cropped by CiteAb from the following publication (https://waojournal.biomedcentral.com/articles/10.1186/s40413-017-0154-3), licensed under a CC-BY license.. Using the HRP format of this antibody.
Applications for Goat anti-Canine IgE Heavy Chain Secondary Antibody
Application
Recommended Usage
ELISA
1:1000 - 1:30000
Immunocytochemistry/ Immunofluorescence
1:200- 1:2000
Immunohistochemistry
1:200- 1:2000
Immunohistochemistry-Paraffin
1:200 - 1:2000
Western Blot
1:1000 - 1:30000
Application Notes
ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using NB7343 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Phosphate Buffered Saline (PBS)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: IgE Heavy Chain
Alternate Names
Constant Region of Heavy Chain of IgE, Ig Epsilon Chain C Region, Ig Epsilon Chain C Region ND, IgE, Immunoglobulin Epsilon, immunoglobulin heavy constant epsilon
Additional IgE Heavy Chain Products
Product Documents for Goat anti-Canine IgE Heavy Chain Secondary Antibody
Product Specific Notices for Goat anti-Canine IgE Heavy Chain Secondary Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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