Goat anti-Canine IgE Heavy Chain Secondary Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NB7346
Conjugate
Catalog #
Key Product Details
Species Reactivity
Canine
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
HRP
Antibody Source
Polyclonal Goat IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Dog IgE
Reactivity Notes
Use in Borreliella burgdorferi reported in secitific publication PMID: 32792093
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with dog IgE. This may cross react with IgE from other species. There was less than 0.01% reactivity with purified dog IgG1, IgG2, IgA and IgM.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Goat anti-Canine IgE Heavy Chain Secondary Antibody [HRP]
Western Blot: Goat anti-Canine IgE Heavy Chain Secondary Antibody [HRP] [NB7346] - D.p. extracts have effects on MCs though the IgE-FcepsilonRI axis. SDS-PAGE of recombinants (300 ng) and Western blotting (168.8 ng, for rDer p 15; 143.8 ng of rDer p 18 per gel lane) of specific IgE in canine sera. Image collected and cropped by CiteAb from the following publication (https://waojournal.biomedcentral.com/articles/10.1186/s40413-017-0154-3), licensed under a CC-BY license.
Applications for Goat anti-Canine IgE Heavy Chain Secondary Antibody [HRP]
Application
Recommended Usage
ELISA
1:10000 - 1:100000
Immunocytochemistry/ Immunofluorescence
1:200 - 1:2000
Immunohistochemistry
1:200 - 1:2000
Western Blot
1:2000 - 1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using NB7346 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Phosphate Buffered Saline (PBS) containing 0.2%BSA
Preservative
0.05% Pro-Clean 400
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: IgE Heavy Chain
Alternate Names
Constant Region of Heavy Chain of IgE, Ig Epsilon Chain C Region, Ig Epsilon Chain C Region ND, IgE, Immunoglobulin Epsilon, immunoglobulin heavy constant epsilon
Additional IgE Heavy Chain Products
Product Documents for Goat anti-Canine IgE Heavy Chain Secondary Antibody [HRP]
Product Specific Notices for Goat anti-Canine IgE Heavy Chain Secondary Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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