Rabbit anti-Feline IgG Fc Secondary Antibody [Alkaline Phosphatase]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72645
Conjugate
Catalog #
Key Product Details
Species Reactivity
Feline
Applications
Dot Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Alkaline Phosphatase
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Cat IgG F(c) fragment
Specificity
No reaction was observed against Cat IgG F(ab).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Cat IgG, Cat IgG F(c) and Cat Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Cat IgG, Cat IgG F(c) and Cat Serum
Scientific Data Images for Rabbit anti-Feline IgG Fc Secondary Antibody [Alkaline Phosphatase]
Dot Blot: Rabbit anti-Feline IgG Fc Secondary Antibody [Alkaline Phosphatase] [NBP1-72645] - Antigen: Cat IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Rabbit anti-Cat IgG Fc Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Blocked with blocking buffer for 60 min at RT.
Applications for Rabbit anti-Feline IgG Fc Secondary Antibody [Alkaline Phosphatase]
Application
Recommended Usage
ELISA
1:4000 - 1:16000
Immunohistochemistry
1:200 - 1:1000
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:500 - 1:2500
Application Notes
This product has been tested by ELISA and dot blot. This product is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Rabbit anti-Feline IgG Fc Secondary Antibody [Alkaline Phosphatase]
Product Specific Notices for Rabbit anti-Feline IgG Fc Secondary Antibody [Alkaline Phosphatase]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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