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Key Product Details

Species Reactivity

Feline

Applications

ELISA, Immunohistochemistry, Western Blot

Label

HRP

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Cat IgG F(c) fragment

Specificity

No reaction was observed against Cat IgG F(ab).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Cat IgG, Cat IgG F(c) and Cat Serum

Scientific Data Images for Rabbit anti-Feline IgG Fc Secondary Antibody [HRP]

ELISA: Rabbit anti-Feline IgG Fc Secondary Antibody [HRP] [NBP1-73582] - ELISA results of purified Rabbit anti-Feline IgG Fc Secondary antibody [HRP] Antibody tested against purified Cat IgG Fc. Each well was coated in duplicate with 1.0 ug of Cat IgG Fc (red line). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate.

Applications for Rabbit anti-Feline IgG Fc Secondary Antibody [HRP]

Application
Recommended Usage

ELISA

1:75000 - 1:150000

Immunohistochemistry

1:500 - 1:2500

Western Blot

1:1000 - 1:10000
Application Notes
This product has been tested by ELISA. This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Gentamicin Sulfate

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Product Documents for Rabbit anti-Feline IgG Fc Secondary Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rabbit anti-Feline IgG Fc Secondary Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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