Rat Anti-Mouse IgG2A APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # F0130
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Rat IgG1 Clone # 344701
Product Specifications
Immunogen
Cocktail of mouse IgG1 and IgG2A
Specificity
The specificity of this antibody was confirmed by direct ELISA on mouse IgG2A monoclonal antibodies, and it does not cross-react with IgG1, IgG2B, IgG3, IgM, IgA, or IgE antibodies. By flow cytometry, this isotype-specific secondary antibody detects primary antibodies of the mouse IgG2A isotype, and does not detect primary antibodies of mouse IgG1, IgG2B, or IgG3 class.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Rat Anti-Mouse IgG2A APC-conjugated Antibody
Detection of Mouse IgG2APrimary Antibody by Flow Cytometry.
Human peripheral blood lymphocytes were stained with A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG2A, filled histogram) or isotype control (Catalog # MAB003, open histogram), or B) Mouse Anti-Human CD8a Monoclonal Antibody (Catalog # MAB1509, Mouse IgG2B, filled histogram) or isotype control (Catalog # MAB003, open histogram), followed by APC-conjugated Rat Anti-Mouse IgG2AIsotype-specific Secondary Antibody (Catalog # F0130). View our protocol for Staining Membrane-associated Proteins.Applications for Rat Anti-Mouse IgG2A APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Mouse IgG2A Primary Antibody
Sample: Mouse IgG2A Primary Antibody
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG2A
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G2A Fc
Additional IgG2A Products
Product Documents for Rat Anti-Mouse IgG2A APC-conjugated Antibody
Product Specific Notices for Rat Anti-Mouse IgG2A APC-conjugated Antibody
For research use only
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