Non-Human Primate IL-1 beta/IL-1F2 Cartridge

Name: Non-Human Primate IL-1 beta/IL-1F2 Cartridge
Brand: ProteinSimple
SKU: SPCKB-NP-011595

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-NP-011595

Simple Plex Non-Human Primate IL-1 beta assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.064 pg/mL
Assay Range:	0.4 - 1,530 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Non-Human Primate IL-1 beta/IL-1F2 Cartridge Summary:	Simple Plex assay for the detection of non-human primate Interleukin 1 beta (IL-1 beta ) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Non-Human Primate IL-1 beta/IL-1F2 Cartridge

Technical Data
Citations

Product Specifications for Non-Human Primate IL-1 beta/IL-1F2 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Non-human Primate
Specificity	Natural and recombinant IL-1 beta.
Cross-reactivity	Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	7	6
Mean (pg/mL)	8.66	446	8.42	410
Standard Deviation	0.427	16.5	0.342	23.3
CV%	4.9	3.7	4	5.7

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=3)	106	104-107
EDTA Plasma (n=4)	98	87-109
Heparin Plasma (n=4)	99	92-106
Serum (n=4)	96	77-108

Linearity

Samples containing and/or spiked with high concentrations of recombinant rhesus IL-1 beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Non-Human Primate IL-1 beta/IL-1F2 Cartridge

	Non-Human Primate IL-1 beta Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 beta/IL-1F2

IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.

Item	Value
Long Name	Interleukin 1 beta
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names	IL-1, IL-1 beta, IL-1F2, IL-1b, IL1 beta, IL1-BETA, IL1B, IL1F2IL-1 beta, catabolin, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Non-Human Primate IL-1 beta/IL-1F2 Cartridge

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Protocols & Manuals

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