Simple Plex Human Dkk-1 Cartridge

Name: Simple Plex Human Dkk-1 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000485

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000485

Product Datasheet / COA

Simple Plex Human DKK-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	3.61 pg/mL
Assay Range:	22.3 - 13,620 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Dkk-1 Cartridge Summary:	Simple Plex assay for the detection of human Dkk-1 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Dkk-1 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Dkk-1 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Dkk-1.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	16	35
Mean (pg/mL)	83.5	3657	80.1	3816
Standard Deviation	5.26	270	7.51	277
CV%	6.3	7.4	9.4	7.3

Linearity

Samples containing and/or spiked with high concentrations of human Dkk-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human Dkk-1 Cartridge

	Human Dkk-1 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Dkk-1

Members of the dickkopf-related protein family (Dkk-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. Dkk-3 and -4 also have one prokineticin domain. Dkk-1, -2, and -4 function as antagonists of canonical Wnt signaling by binding to LRP5/6, preventing interaction of LRP5/6 with Wnt-Frizzled complexes. Dkk-1, -2, and -4 also bind cell surface Kremen-1 or -2 and promote the internalization of LRP5/6. Antagonistic activity of Dkk-3 has not been demonstrated. Dkk proteins have distinct patterns of expression in adult and embryonic tissues and have a wide range of effects on tissue development and morphogenesis. The Dkk family also includes Soggy, which is homologous to Dkk-3 but not to the other family members. Soggy has not been shown to inhibit Wnt signaling, and its role in the pathway is unclear.

Item	Value
Long Name	Dickkopf-1
Entrez Gene IDs	22943 (Human); 13380 (Mouse); 102123735 (Cynomolgus Monkey)
Alternate Names	Dickkopf-1, Dkk-1, Dkk1, SKdickkopf-1 like, dickkopf (Xenopus laevis) homolog 1, dickkopf homolog 1 (Xenopus laevis), dickkopf related protein-1, dickkopf-related protein 1, hDkk-1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Dkk-1 Cartridge

Product Datasheets

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COA

Protocols & Manuals

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