Simple Plex Human Fas Ligand Cartridge

Name: Simple Plex Human Fas Ligand Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-004009

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-004009

Simple Plex Human Fas Ligand/TNFSF6 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.523 pg/mL
Assay Range:	0.868 - 3,312 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Fas Ligand Cartridge Summary:	Simple Plex assay for the detection of human Fas Ligand in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Fas Ligand Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Fas Ligand Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Fas Ligand
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	1
n	16	16	29	29
Mean (pg/mL)	25.7	1226	1286	27.2
Standard Deviation	1.24	55.3	103	1.95
CV%	4.8	4.5	8	7.2

Linearity

Samples containing and/or spiked with high concentrations of human Fas Ligand were serially diluted with Sample Diluent to produce samples within the range of the assay.

Human Fas Ligand Simple Plex Assay Standard Curve

Scientific Data Examples for Simple Plex Human Fas Ligand Cartridge

	Human Fas Ligand Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Fas Ligand/TNFSF6

Fas Ligand (FasL), also known as CD178, CD95L, or TNFSF6, is a transmembrane protein homotrimer that binds to Fas/CD95 and triggers apoptosis in the Fas-expressing cell. Fas Ligand also binds the soluble decoy receptor DcR3. It is expressed on activated Th1 cells, CD8+ cytotoxic T cells, and NK cells. A soluble form can be released which remains trimeric and retains the ability to bind Fas. Fas Ligand-induced apoptosis plays a central role in the development of immune tolerance and the maintance of immune privileged sites. Tumor cells can evade immune surveillance by upregulating Fas Ligand to kill tumor infiltrating lymphocytes. In gld mice, a Fas Ligand point mutation is the cause of severe lymphoproliferation and systemic autoimmunity.

Item	Value
Entrez Gene IDs	356 (Human); 14103 (Mouse); 25385 (Rat)
Alternate Names	APT1LG1CD95L, APTL, Apoptosis antigen ligand, CD178, CD178 antigen, CD95-L, CD95L, FASLCD95 ligand, FASLG, Fas Ligand, Fas antigen ligand, Fas ligand (TNF superfamily, member 6), TNFSF6, TNFSF6FasL, apoptosis (APO-1) antigen ligand 1, tumor necrosis factor (ligand) superfamily, member 6, tumor necrosis factor ligand superfamily member 6

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Fas Ligand Cartridge

Product Datasheets

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Protocols & Manuals

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