Simple Plex Human HVEM/TNFRSF14 Cartridge

Name: Simple Plex Human HVEM/TNFRSF14 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-003550

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-003550

Simple Plex Human HVEM/TNFRSF14 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)
Sensitivity:	0.12 pg/mL
Assay Range:	1.7 - 16,240 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human HVEM/TNFRSF14 Cartridge Summary:	Simple Plex assay for the detection of human HVEM in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human HVEM/TNFRSF14 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human HVEM/TNFRSF14 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human HVEM.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	34	34
Mean (pg/mL)	96.8	4590	95.2	4678
Standard Deviation	8.14	348	9.93	365
CV%	8.4	7.6	10.4	7.8

Linearity

Samples containing and/or spiked with high concentrations of human HVEM were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human HVEM/TNFRSF14 Cartridge

	Human HVEM/TNFRSF14 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HVEM/TNFRSF14

HVEM/TNFRSF14 (herpesvirus entry mediator) is a transmembrane protein that is most highly expressed on naïve, memory, and regulatory T cells but declines during T cell activation. It is present at low levels on most resting leukocytes. HVEM is a receptor for BTLA, CD160, and LIGHT. A cis interaction between HVEM and BTLA promotes cell survival, while trans interaction promotes a state of lymphocyte inactivity. LIGHT is expressed following T cell activation and competes with BTLA for HVEM binding, leading to a proinflammatory response. The herpesvirus envelope glycoprotein gD, which binds HVEM to initiate membrane fusion, can antagonize both BTLA and LIGHT binding. Human, but not mouse, HVEM can also bind lymphotoxin-alpha.

Item	Value
Long Name	Herpesvirus Entry Mediator
Entrez Gene IDs	8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)
Alternate Names	ATAR, CD270, CD270 antigen, CD40-like protein, HVEM, HVEMTR2HVEAATAR, Herpes virus entry mediator A, Herpesvirus entry mediator A, HveA, LIGHTR, TNFRSF14, Tumor necrosis factor receptor-like 2, tumor necrosis factor receptor superfamily member 14, tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator), tumor necrosis factor receptor-like gene2

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human HVEM/TNFRSF14 Cartridge

Product Datasheets

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Protocols & Manuals

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