Simple Plex Human ICAM-1/CD54 Cartridge

Name: Simple Plex Human ICAM-1/CD54 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000303

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000303

Simple Plex Human ICAM-1/CD54 assay kit for use on Ella instrument. Contains cartridge, sample diluent 000303, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (500 nL), EDTA Plasma (500 nL), Heparin Plasma (500 nL), Cerebrospinal Fluid (5 uL)
Sensitivity:	2.01 pg/mL
Assay Range:	4.1 - 15,630 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Simple Plex Human ICAM-1/CD54 Cartridge Summary:	Simple Plex assay for the detection of human ICAM-1 in serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human ICAM-1/CD54 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human ICAM-1/CD54 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human ICAM-1.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	10	10
Mean (pg/mL)	117	5027	106	5096
Standard Deviation	6.6	318	6.78	245
CV%	5.7	6.3	6.4	4.8

Linearity

Samples containing and/or spiked with high concentrations of human ICAM-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human ICAM-1/CD54 Cartridge

	Human ICAM-1 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ICAM-1/CD54

ICAM-1 (intercellular adhesion molecule-1), also known as CD54, is a transmembrane protein that is upregulated on endothelial and epithelial cells at sites of inflammation. It mediates the vascular adhesion and paracellular migration of leukocytes expressing activated LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). It also binds several non-integrin ligands including CD43/Sialophorin, Fibrinogen, Hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes. Soluble ICAM-1 promotes angiogenesis and serves an indicator of vascular endothelial cell activation or damage. Elevated levels of soluble ICAM-1 are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, increased abdominal fat mass, hypertension, liver disease, certain malignancies, and cerebral malaria.

Item	Value
Long Name	Intercellular Adhesion Molecule 1
Entrez Gene IDs	3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names	BB2, CD54, CD54 antigen, ICAM-1, ICAM1, Major group rhinovirus receptor, P3.58, cell surface glycoprotein P3.58, human rhinovirus receptor, intercellular adhesion molecule 1, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human ICAM-1/CD54 Cartridge

Product Datasheets

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Protocols & Manuals

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