Simple Plex Human IL-10 Cartridge

Name: Simple Plex Human IL-10 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000276

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000276

Citations (1)

Product Datasheet / COA

This product is in the process of discontinuation.

Simple Plex Human IL-10 is in the process of discontinuation and is no longer available on our stocked program. We have replaced it with the Human IL-10 2nd gen assay in panel SPCKB-PS-009385.

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.14 pg/mL
Assay Range:	0.58 - 2,212 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human IL-10 Cartridge Summary:	Simple Plex assay for the detection of human Interleukin 10 (IL-10) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human IL-10 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human IL-10 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human IL-10.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	6	6
Mean (pg/mL)	33	1591	33.2	1681
Standard Deviation	1.55	95.1	2.43	120
CV%	4.6	6	7.1	7.1

Recovery

Recovery at three different spiked concentrations withinthe range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=6)	105	88-113
Heparin Plasma (n=6)	102	89-118
Serum (n=6)	94	88-98

Linearity

Samples containing and/or spiked with high concentrations of human IL-10 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human IL-10 Cartridge

	Human IL-10 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-10

Interleukin-10 functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.

Item	Value
Long Name	Interleukin 10
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)
Alternate Names	CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL-10, IL-10MGC126451, IL10, IL10A, TGIF, interleukin 10, interleukin-10

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Product Documents for Simple Plex Human IL-10 Cartridge

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

Download Protocol