Simple Plex Human IL-11 Cartridge

Name: Simple Plex Human IL-11 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000648

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000648

Product Datasheet / COA

Simple Plex Human IL-11 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	2 pg/mL
Assay Range:	11.7 - 44,600 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human IL-11 Cartridge Summary:	Simple Plex assay for the detection of human IL-11 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human IL-11 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human IL-11 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human IL-11.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	64	64
Mean (pg/mL)	306	15605	313	14627
Standard Deviation	10	1173	22.1	1282
CV%	3.3	7.5	7	8.8

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	94	88-101
Heparin Plasma (n=4)	94	83-104
Serum (n=4)	101	95-106

Linearity

Samples containing and/or spiked with high concentrations of human IL-11 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human IL-11 Cartridge

	Human IL-11 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-11

Interleukin-11, also known as AGIF, is a pleiotropic cytokine in the IL-6 family, which also includes LIF, CNTF, Oncostatin M, Cardiotrophin-1, IL-27, and IL-31. It is secreted by osteoblasts, synoviocytes, fibroblasts, chondrocytes, intestinal myofibroblasts, and trophoblasts. It is found in the plasma mainly during inflammation, and is considered to be primarily anti-inflammatory. It stimulates hematopoiesis and thrombopoiesis, regulates macrophage differentiation, and confers mucosal protection in the intestine. It has also been found to enhance T cell polarization toward Th2, promote B cell IgG production, increase osteoclast bone absorption, protect endothelial cells from oxidative stress, and regulate epithelial proliferation and apoptosis. IL-11 signals through a receptor complex that contains IL-11 R alpha and gp130. A soluble form of IL-11 R alpha can bind IL-11 and either form a signaling complex with gp130 on the cell surface or inhibit cell surface IL-11 R alpha/gp130 signaling.

Item	Value
Long Name	Interleukin 11
Entrez Gene IDs	3589 (Human); 16156 (Mouse); 171040 (Rat); 102128313 (Cynomolgus Monkey)
Alternate Names	AGIF, AGIFoprelvekin, Adipogenesis inhibitory factor, IL-11, IL-11Oprelvekin, IL11, Oprelvekin, interleukin 11, interleukin-11

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human IL-11 Cartridge

Product Datasheets

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COA

Protocols & Manuals

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