Simple Plex Human Serpin E1/PAI-1 Cartridge

Name: Simple Plex Human Serpin E1/PAI-1 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000342

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000342

Simple Plex Human Serpin E1/PAI-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD06, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	0.652 pg/mL
Assay Range:	2.32 - 8,870 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Serpin E1/PAI-1 Cartridge Summary:	Simple Plex assay for the detection of human Serpin E1 in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Serpin E1/PAI-1 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Serpin E1/PAI-1 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Serpin E1.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	21	21
Mean (pg/mL)	88.5	4428	91.5	4346
Standard Deviation	5.16	366	9.65	366
CV%	5.8	8.3	10.5	8.4

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	108	105-111

Linearity

Samples containing and/or spiked with high concentrations of human Serpin E1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human Serpin E1/PAI-1 Cartridge

	Human Serpin E1 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Serpin E1/PAI-1

The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.

Item	Value
Long Name	Plasminogen Activator Inhibitor
Entrez Gene IDs	5054 (Human); 18787 (Mouse)
Alternate Names	Endothelial plasminogen activator inhibitor, Nexin, PAI-1, PAI1, PAI1PAI-1, PAISerpin E1, PLANH1, PLANH1plasminogen activator inhibitor 1, Serpin E1, serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Serpin E1/PAI-1 Cartridge

Product Datasheets

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Protocols & Manuals

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