AG 490

Tocris Bioscience, part of Bio-Techne | Catalog # 0414

COA / SDS

EGFR-kinase inhibitor. Also JAK2, JAK3 inhibitor

Key Product Details

Description:	EGFR-kinase inhibitor. Also JAK2, JAK3 inhibitor
Alternative Names:	Tyrphostin AG 490
Chemical Name:	(E)-2-Cyano-3-(3,4-dihydrophenyl)-N-(phenylmethyl)-2-propenamide
Purity:	≥99% (HPLC)
Molecular Weight:	294.31

View Full Technical Details for AG 490

Technical Data
Citations

Biological Activity for AG 490

AG 490 is a selective inhibitor of EGF receptor tyrosine kinase (IC₅₀ values are 2 and 13.5 μM for EGFR and ErbB2 respectively). Inhibitor of JAK2, JAK3/STAT, JAK3/AP-1 and JAK3/MAPK pathways and potently inhibits cytokine-independent cell growth in vitro and tumor cell invasion in vivo.

Technical Data for AG 490

M. Wt	294.31
Formula	C₁₇H₁₄N₂O₃
Storage	Store at RT
Purity	≥99% (HPLC)
CAS Number	133550-30-8
PubChem ID	5328779
InChi Key	TUCIOBMMDDOEMM-RIYZIHGNSA-N
Smiles	OC1=C(O)C=C(\C=C(/C#N)C(=O)NCC2=CC=CC=C2)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for AG 490

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	ethanol	5.89	20
	DMSO	29.43	100

Preparing Stock Solutions for AG 490

The following data is based on the product molecular weight 294.31.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.40 mL	16.99 mL	33.98 mL
5 mM	0.68 mL	3.40 mL	6.80 mL
10 mM	0.34 mL	1.70 mL	3.40 mL
50 mM	0.07 mL	0.34 mL	0.68 mL

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Reconstitution Calculator

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References for AG 490

References are publications that support the biological activity of the product.

Wang JAK3, STAT, and MAPK signaling pathways as novel molecular targets for the tyrphostin AG-490 regulation of IL-2-mediated T cell response. J.Immunol. 1999 PMID: 10201908
Gazit Tyrphostins. 2. Heterocyclic and α-substituted benzylidenemalononitrile tyrophostins as potent inhibitors of EGF receptor and ErbB2/neu tyrosine kinases. J.Med.Chem. 1991 PMID: 1676428
Meydan Inhbition of acute lymphoblastic leukaemia by a JAK-2 inhibitor. Nature 1996 PMID: 8628398
Abe The polycythemia vera-associated Jak2 V617F mutant induces tumorigenesis in nude mice. Int.Immunopharmacol. 2009 PMID: 19327411