AMG 900

Name: AMG 900
Brand: Tocris Bioscience
SKU: 7865

Tocris Bioscience, part of Bio-Techne | Catalog # 7865

COA / SDS

Potent pan-Aurora kinase inhibitor

Key Product Details

Description:	Potent pan-Aurora kinase inhibitor
Chemical Name:	N-[4-[[3-(2-Amino-4-pyrimidinyl)-2-pyridinyl]oxy]phenyl]-4-(4-methyl-2-thienyl)-1-phthalazinamine
Purity:	≥98% (HPLC)
Molecular Weight:	503.58

View Full Technical Details for AMG 900

Technical Data
Citations

Biological Activity for AMG 900

AMG 900 is a potent pan-Aurora kinase inhibitor (IC₅₀ values are 1 nM, 4 nM, and 5 nM for Aurora C, B, and A, respectively). It is more than 10-fold selective for Aurora kinases over JNK2, Tie2, p38α, Met, and TYK2. AMG 900 inhibits autophosphorylation of Aurora A and B, as well as the phosphorylation of histone H3 on Ser in HeLa cells. AMG 900 inhibits proliferation of 26 tumor cell lines (EC₅₀ ranges from 0.7 nM - 5.3 nM), including Paclitaxel (Cat. No. 1097)-resistant tumor cell lines. In an HCT116 tumor xenograft model, AMG 900 inhibits phosphorylation of histone H3 and tumor growth. It is orally bioavailable.

Technical Data for AMG 900

M. Wt	503.58
Formula	C₂₈H₂₁N₇OS
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	945595-80-2
PubChem ID	24856041
InChi Key	IVUGFMLRJOCGAS-UHFFFAOYSA-N
Smiles	N1=NC(C=2SC=C(C2)C)=C3C=CC=CC3=C1NC4=CC=C(OC=5N=CC=CC5C6=NC(=NC=C6)N)C=C4

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for AMG 900

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	5.04	10 with gentle warming

Preparing Stock Solutions for AMG 900

The following data is based on the product molecular weight 503.58.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	19.86 mL	99.29 mL	198.58 mL
0.5 mM	3.97 mL	19.86 mL	39.72 mL
1 mM	1.99 mL	9.93 mL	19.86 mL
5 mM	0.40 mL	1.99 mL	3.97 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for AMG 900

References are publications that support the biological activity of the product.

Payton Preclinical evaluation of AMG 900, a novel potent and highly selective pan-aurora kinase inhibitor with activity in taxane-resistant tumor cell lines. Cancer Res. 2010 PMID: 20935223