CAF 382

Name: CAF 382
Brand: Tocris Bioscience
SKU: 8105

Tocris Bioscience, part of Bio-Techne | Catalog # 8105

COA / SDS

Potent and selective CDKL5 inhibitor

Key Product Details

Description:	Potent and selective CDKL5 inhibitor
Chemical Name:	N-[5-[(5-Propan-2-yl-1,3-oxazol-2-yl)methylsulfanyl]-1,3-thiazol-2-yl]piperidine-4-carboxamide trifluoroacetate
Purity:	≥98% (HPLC)
Molecular Weight:	480.52

View Full Technical Details for CAF 382

Technical Data
Citations

Biological Activity for CAF 382

CAF 382 is potent and selective CDKL5 (cyclin-dependent kinase-like 5) inhibitor (IC₅₀ = 10 nM). Exhibits nearly 100-fold selectivity for CDKL5 over CDKL2, low inhibition for CDKL3 and CDKL4 (IC₅₀ = 2.1-2.7 µM), and no inhibition for CDKL1. CAF 382 lacks GSK3β activity. It causes a significant reduction in pSer222 EB2 at 5 nM without a change in total EB2 levels in rat primary cortical neuron cultures. CAF 382 is an analog of SNS 032 (Cat. No. 4075).

Licensing Information

This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the SGC-CAF382-1 probe summary on the SGC website.

Technical Data for CAF 382

M. Wt	480.52
Formula	C₁₆H₂₂N₄O₂S₂.CF₃CO₂H
Storage	Store at -20°C
Purity	≥98% (HPLC)
InChi Key	CZYALDCQIWYWJJ-UHFFFAOYSA-N
Smiles	O=C(C1CCNCC1)NC2=NC=C(SCC3=NC=C(C(C)C)O3)S2.O=C(O)C(F)(F)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for CAF 382

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	48.05	100
	ethanol	9.61	20 with gentle warming

Preparing Stock Solutions for CAF 382

The following data is based on the product molecular weight 480.52.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.08 mL	10.41 mL	20.81 mL
5 mM	0.42 mL	2.08 mL	4.16 mL
10 mM	0.21 mL	1.04 mL	2.08 mL
50 mM	0.04 mL	0.21 mL	0.42 mL

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References for CAF 382

References are publications that support the biological activity of the product.

Castano Discovery and characterization of a specific inhibitor of serine-threonine kinase cyclin-dependent kinase like 5 (CDKL5) demonstrates role in hippocampal CA1 physiology. Elife 2023 PMID: 37490324