CoraFluor™ 2, Haloalkane

Name: CoraFluor™ 2, Haloalkane
Brand: Tocris Bioscience
SKU: 8818

Tocris Bioscience, part of Bio-Techne | Catalog # 8818

COA / SDS

Cell permeable terbium cryptate FRET donor for TR-FRET assay development, with chloroalkane ligand

Key Product Details

Description:	Cell permeable terbium cryptate FRET donor for TR-FRET assay development, with chloroalkane ligand
Molecular Weight:	1662.7

View Full Technical Details for CoraFluor™ 2, Haloalkane

Technical Data
Citations

Biological Activity for CoraFluor™ 2, Haloalkane

CoraFluor™ 2, Haloalkane is a cell permeable terbium-based time-resolved Förster resonance energy transfer (TR-FRET) donor for live cell TR-FRET assay development, such as target engagement and ternary complex assays. CoraFluor™ 2, Haloalkane is designed with a chloroalkane ligand for reactivity with haloalkane dehalogenase labeled proteins. CoraFluor™ 2, Haloalkane can be used in live cell intracellular TR-FRET assays. It is exceptionally bright, providing unmatched assay sensitivity and stability in biological media over other commercially available TR-FRET donors. CoraFluor™ 2, Haloalkane displays red shifted excitation and is excitable by both 365 nm and 405 nm lasers. It is compatible with common acceptor dyes such as FAM/FITC, AF488, GFP, mCherry, TMR and Cyanine 5, SE (Cat. No. 5436), enabling seamless assay integration in high throughput screening applications.

CoraFluor™ 1, Haloalkane, CoraFluor™ 1, thiol reactive, CoraFluor™ 1, amine reactive and CoraFluor™ 2, amine reactive also available.

For more information, please see our CoraFluor™ TR-FRET technology flyer .

CoraFluor is a trademark of Bio-Techne Corp.

Licensing Information

Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Optical Data for CoraFluor™ 2, Haloalkane

λ_abs	356 nm
Extinction Coefficient (ε)	15900 M^-1cm^-1
Quantum Yield (φ)	0.35
Closest Laser line	337 nm
Cell Permeable	Yes
Application	Time-resolved fluorescence energy transfer (TR-FRET)

Scientific Data Examples for CoraFluor™ 2, Haloalkane

	Live cell TR-FRET assay. Cellular dose-dependent inhibition of HDAC1-dehalogenase fusion protein labeled with CoraFluor™ 2, Haloalkane in HEK293T cells by various HDAC inhibitors (treatment for 4 h at 37 °C, 1 μM SAHA-NCT).

Technical Data for CoraFluor™ 2, Haloalkane

M. Wt	1662.7
Storage	Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

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References for CoraFluor™ 2, Haloalkane

References are publications that support the biological activity of the product.

Payne Bright and stable luminescent probes for target engagement profiling in live cells. Nat.Chem.Biol. 2021 PMID: 34675420