DAPT

Name: DAPT
Brand: Tocris Bioscience
SKU: 2634

Tocris Bioscience, part of Bio-Techne | Catalog # 2634

(6)

Citations (347)

COA / SDS

γ-secretase inhibitor; induces neuronal differentiation; blocks Notch signaling

Key Product Details

Chemical Name:	(2S)-N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine 1,1-dimethylethyl ester
Purity:	≥99%

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Technical Data
Citations / Reviews (6)

Biological Activity for DAPT

DAPT is a γ-secretase inhibitor. DAPT reduces Aβ40 and Aβ42 levels in human primary neuronal cultures (IC₅₀ values are 115 and 200 nM for total Aβ and Aβ42 respectively) and in brain extract, cerebrospinal fluid and plasma in vivo. DAPT has no effect on APPα and APPβ levels. DAPT blocks Notch signaling in hybrid human-mouse fetal thymus organ culture (FTOC) and causes ESCs to commit to neuronal differentiation. DAPT can be used in a small molecule cocktail to derive cortical neurons from hPSCs and to maintain hepatocytes in culture. DAPT also promotes the formation of cone photoreceptors in retinal organoids.

For more information about how DAPT may be used, see our protocols: Accelerated Induction of Cortical Neurons from hiPSCs, Human iPSC-derived Myoblasts, Generating Midbrain Dopaminergic Neurons from hPSCs.

DAPT synthesized to Ancillary Material Grade also available.

Scientific Data Examples for DAPT

	Dopaminergic Neurons Derived from hiPSCs using DAPT. Dopaminergic neurons derived from human induced pluripotent stem cells (hiPSC) using SB 431542 (Catalog # 1614, Tocris), CHIR 99021 (Catalog # 4423, Tocris), DAPT and Purmorphamine (Catalog # 4551, Tocris). Cells cultured in Neuronal Media supplemented with TGF-3 (Catalog # 8420-B3, R&D Systems), cAMP, GDNF (Catalog # 212-GD, R&D Systems), BDNF (Catalog # 248-BDB, R&D Systems), N21-MAX (Catalog # AR008, R&D Systems). Differentiation shown at days 55 (left) and 62 (right). Images courtesy of Kevin Flynn, Bio-Techne.

Technical Data for DAPT

Formula	C₂₃H₂₆F₂N₂O₄
Storage	Store at +4°C
Purity	≥99%
CAS Number	208255-80-5

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for DAPT

Solubility

Soluble Soluble to 100 mM in DMSO

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References for DAPT

References are publications that support the biological activity of the product.

Wu Z, Rowe R, Lim K, Lin Y, Willis A, Tang Y, Li X, Nor J, Maillard I, Weiss S A Snail1/Notch1 signalling axis controls embryonic vascular development. Nat Commun, 2014;5(0):3998. 2014 PMID: 24894949
J Högström, S Heino, P Kallio, M Lähde, VM Leppänen, D Balboa, Z Wiener, K Alitalo Transcription factor PROX1 suppresses Notch pathway activation via the nucleosome remodeling and deacetylase complex in colorectal cancer stem-like cells Cancer Res., 2018;0(0):. 2018 PMID: 30154153
Benner, Eric J, Luciano, Dominic, Jo, Rebecca, Abdi, Khadar, Paez-Gonzalez, Patricia, Sheng, Huaxin, Warner, David S, Liu, Chunlei, Eroglu, Cagla, Kuo, Chay T Protective astrogenesis from the SVZ niche after injury is controlled by Notch modulator Thbs4. Nature, 2013;497(7449):369-73. 2013 PMID: 23615612
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J Ezpeleta, F Boudet-Dev, M Pietri, A Baudry, V Baudouin, A Alleaume-B, N Dagoneau, O Kellermann, JM Launay, B Schneider Protective role of cellular prion protein against TNF?-mediated inflammation through TACE ?-secretase Sci Rep, 2017;7(1):7671. 2017 PMID: 28794434
Wang, Yidong, Wu, Bingruo, Chamberlain, Alyssa A, Lui, Wendy, Koirala, Pratisth, Susztak, Katalin, Klein, Diana, Taylor, Verdon, Zhou, Bin Endocardial to myocardial notch-wnt-bmp axis regulates early heart valve development. PLoS ONE, 2013;8(4):e60244. 2013 PMID: 23560082
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N Zeltner, F Fattahi, NC Dubois, N Saurat, F Lafaille, L Shang, B Zimmer, J Tchieu, MA Soliman, G Lee, JL Casanova, L Studer Capturing the biology of disease severity in a PSC-based model of familial dysautonomia Nat. Med., 2016;22(12):1421-1427. 2016 PMID: 27841875
Edri, Reuven, Yaffe, Yakey, Ziller, Michael, Mutukula, Naresh, Volkman, Rotem, David, Eyal, Jacob-Hirsch, Jasmine, Malcov, Hagar, Levy, Carmit, Rechavi, Gideon, Gat-Viks, Irit, Meissner, Alexande, Elkabetz, Yechiel Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors. Nat Commun, 2015;6(0):6500. 2015 PMID: 25799239
Diaz B, Yuen A, Iizuka S, Higashiyama S, Courtneidge S Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia. J Cell Biol, 2013;201(2):279-92. 2013 PMID: 23589494
MA Schumacher, M Hedl, C Abraham, JK Bernard, PR Lozano, JJ Hsieh, D Almohazey, EB Bucar, S Punit, PJ Dempsey, MR Frey ErbB4 signaling stimulates pro-inflammatory macrophage apoptosis and limits colonic inflammation Cell Death Dis, 2017;8(2):e2622. 2017 PMID: 28230865
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A Fiorenzano, E Sozzi, M Birtele, J Kajtez, J Giacomoni, F Nilsson, A Bruzelius, Y Sharma, Y Zhang, B Mattsson, J Emnéus, DR Ottosson, P Storm, M Parmar Single-cell transcriptomics captures features of human midbrain development and dopamine neuron diversity in brain organoids Nature Communications, 2021;12(1):7302. 2021 PMID: 34911939
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Q Yu, Z Xia, EC Liong, GL Tipoe Chronic aerobic exercise improves insulin sensitivity and modulates Nrf2 and NF?kappaB/IkappaBalpha pathways in the skeletal muscle of rats fed with a high fat diet Mol Med Rep, 2019;20(6):4963-4972. 2019 PMID: 31702809
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Crawford and Roelink The notch response inhibitor DAPT enhances neuronal differentiation in embryonic stem cell-derived embryoid bodies independently of sonic hedgehog signaling. Dev.Dyn., 2007;236:886 PMID: 17295317
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