Purmorphamine

Name: Purmorphamine
Brand: Tocris Bioscience
SKU: 4551

Tocris Bioscience, part of Bio-Techne | Catalog # 4551

(3)

Citations (105)

COA / SDS

Smo receptor agonist

Key Product Details

Chemical Name:	9-Cyclohexyl-N-[4-(4-morpholinyl)phenyl]-2-(1-naphthalenyloxy)-9H-purin-6-amine
Purity:	≥98%

View Full Technical Details for Purmorphamine

Technical Data
Citations / Reviews (3)

Biological Activity for Purmorphamine

Purmorphamine is a smoothened (Smo) receptor agonist (EC₅₀ ~ 1 μM) and activates the Hedgehog pathway. Purmorphamine induces osteogenesis in mouse mesenchymal progenitor cells (C3H10T1/2). When combined with BMP-4, Purmorphamine can transdifferentiate pre-adipocytes (3T3-L1) and myoblasts (C2C12) into osteoblasts. Purmorphamine induces differentiation of multipotent mesenchymal progenitor cells into osteoblasts, and of spinal motor neurons from pluripotent human stem cells, and protects dopaminergic neurons in a mouse model of Parkinson's disease.

Scientific Data Examples for Purmorphamine

	Applications of Purmorphamine in Neurons Derived from hiPSCs Dopaminergic neurons derived from human induced pluripotent stem cells (hiPSC) using SB 431542 (Catalog # 1614, Tocris), CHIR 99021 (Catalog # 4423, Tocris), DAPT (Catalog # 2634, Tocris) and Purmorphamine. Cells cultured in Neuronal Media supplemented with TGF-3 (Catalog # 8420-B3, R&D Systems), cAMP, GDNF (Catalog # 212-GD, R&D Systems), BDNF (Catalog # 248-BDB, R&D Systems), N21-MAX (Catalog # AR008, R&D Systems). Differentiation shown at days 55 (left) and 62 (right). Images courtesy of Kevin Flynn, Bio-Techne.

Technical Data for Purmorphamine

Formula	C₃₁H₃₂N₆O₂
Storage	Store at -20°C
Purity	≥98%
CAS Number	483367-10-8

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Purmorphamine

Solubility

Soluble Soluble to 100 mM in DMSO

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References for Purmorphamine

References are publications that support the biological activity of the product.

C Lang, KR Campbell, BJ Ryan, P Carling, M Attar, J Vowles, OV Perestenko, R Bowden, F Baig, M Kasten, MT Hu, SA Cowley, C Webber, R Wade-Marti Single-Cell Sequencing of iPSC-Dopamine Neurons Reconstructs Disease Progression and Identifies HDAC4 as a Regulator of Parkinson Cell Phenotypes Cell Stem Cell, 2018;0(0):. 2018 PMID: 30503143
Wu et al. Purmorphamine induces osteogenesis by activation of the hedgehog signaling pathway. Chem.Biol., 2004;11:1229 PMID: 15380183
Sinha et al. Purmorphamine activates the Hedgehog pathway by targeting Smoothened. Nat.Chem.Biol., 2006;2:29 PMID: 16408088
Wu et al. A small molecule with osteogenesis-inducing activity in multipotent mesenchymal progenitor cells. J.Am.Chem.Soc., 2002;124:14520 PMID: 12465946