(±)-Blebbistatin

Name: (±)-Blebbistatin
Brand: Tocris Bioscience
SKU: 1760

Tocris Bioscience, part of Bio-Techne | Catalog # 1760

Citations (271)

COA / SDS

Selective inhibitor of myosin II ATPase activity

Key Product Details

Description:	Selective inhibitor of myosin II ATPase activity
Chemical Name:	(±)-1,2,3,3a-Tetrahydro-3a-hydroxy-6-methyl-1-phenyl-4H-pyrrolo[2,3-b]quinolin-4-one
Purity:	≥99% (HPLC)
Molecular Weight:	292.34

View Full Technical Details for (±)-Blebbistatin

Technical Data
Citations

Biological Activity for (±)-Blebbistatin

(±)-Blebbistatin is a selective inhibitor of myosin II ATPase activity; inhibits contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. (±)-Blebbistatin rapidly and reversibly blocks cell blebbing, and disrupts directed cell migration and cytokinesis in vertebrate cells. (±)-Blebbistatin inhibits arterial thrombosis development, reduces infiltration of inflammatory cells, and reduces damage to vascular tissue in vivo in a mice model of arterial thrombosis.

Active and Inactive Enantiomer also available.

Technical Data for (±)-Blebbistatin

M. Wt	292.34
Formula	C₁₈H₁₆N₂O₂
Storage	Store at +4°C
Purity	≥99% (HPLC)
CAS Number	674289-55-5
PubChem ID	3476986
InChi Key	LZAXPYOBKSJSEX-UHFFFAOYSA-N
Smiles	CC1=CC=C2N=C3N(CCC3(O)C(=O)C2=C1)C1=CC=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for (±)-Blebbistatin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	29.23	100

Preparing Stock Solutions for (±)-Blebbistatin

The following data is based on the product molecular weight 292.34.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.42 mL	17.10 mL	34.21 mL
5 mM	0.68 mL	3.42 mL	6.84 mL
10 mM	0.34 mL	1.71 mL	3.42 mL
50 mM	0.07 mL	0.34 mL	0.68 mL

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Reconstitution Calculator

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References for (±)-Blebbistatin

References are publications that support the biological activity of the product.

Wang Myosin IIA-related actomyosin contractility mediates oxidative stress-induced neuronal apoptosis. Front Mol.Neurosci. 2017 PMID: 28352215
Chen Blebbistatin modulates prostatic cell growth and contrapctility through myosin II signaling. Clin.Sci. 2018 PMID: 30279228
Gao Blebbistatin inhibits neomycin-induced apoptosis in hair cell-like HEI-OC-1 cells and in cochlear hair cells. Front.Cell Neurosci. 2020 PMID: 32116554
Lang Inhibition of suicidal erythrocyte death by blebbistatin Am.J.Physiol.Cell Physiol. 2011 PMID: 21593446
Zhang An inhibitor of myosin II, blebbistatin, suppresses development of arterial thrombosis. Biomed.Pharmacother 2020 PMID: 31918291
Walker Non-muscle myosin II regulates survival threshold of pluripotent stem cells. Nat.Commun. 2010 PMID: 20842192
Straight Dissecting cytokinesis with small molecules. Amer.Soc.Cell Biol.Abstr.
Straight Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor. Science 2003 PMID: 12637748
Limouze Specificity of blebbistatin, an inhibitor of myosin II. J.Muscle Res.Cell Motil. 2004 PMID: 15548862