(S)-(-)-Blebbistatin

Name: ( S )-(-)-Blebbistatin
Brand: Tocris Bioscience
SKU: 1852

Tocris Bioscience, part of Bio-Techne | Catalog # 1852

Citations (22)

COA / SDS

Selective inhibitor of myosin II ATPase activity. Active enantiomer of (±)-blebbistatin (Cat. No. 1760)

Key Product Details

Description:	Selective inhibitor of myosin II ATPase activity. Active enantiomer of (±)-blebbistatin (Cat. No. 1760)
Chemical Name:	(3aS)-(-)-1,2,3,3a-Tetrahydro-3a-hydroxy-6-methyl-1-phenyl-4H-pyrrolo[2,3-b]quinolin-4-one
Purity:	≥99% (HPLC)
Molecular Weight:	292.34

View Full Technical Details for (S)-(-)-Blebbistatin

Technical Data
Citations

Biological Activity for (S)-(-)-Blebbistatin

(S)-(-)-Blebbistatin is a selective inhibitor of myosin II ATPase activity (IC₅₀ ~0.5 - 5 μM); active enantiomer of (±)-blebbistatin. Inhibits contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. (S)-(-)-Blebbistatin rapidly and reversibly blocks cell blebbing, and disrupts directed cell migration and cytokinesis in vertebrate cells.

Inactive Enantiomer and Racemate also available.

Technical Data for (S)-(-)-Blebbistatin

M. Wt	292.34
Formula	C₁₈H₁₆N₂O₂
Storage	Store at +4°C
Purity	≥99% (HPLC)
CAS Number	856925-71-8
PubChem ID	5287792
InChi Key	LZAXPYOBKSJSEX-GOSISDBHSA-N
Smiles	O=C2[C@@]3(O)C(N(C4=CC=CC=C4)CC3)=NC1=CC=C(C)C=C12

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for (S)-(-)-Blebbistatin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	29.23	100

Preparing Stock Solutions for (S)-(-)-Blebbistatin

The following data is based on the product molecular weight 292.34.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.42 mL	17.10 mL	34.21 mL
5 mM	0.68 mL	3.42 mL	6.84 mL
10 mM	0.34 mL	1.71 mL	3.42 mL
50 mM	0.07 mL	0.34 mL	0.68 mL

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References for (S)-(-)-Blebbistatin

References are publications that support the biological activity of the product.

Straight Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor. Science 2003 PMID: 12637748
Straight Dissecting cytokinesis with small molecules. Amer.Soc.Cell Biol.Abstr.
Limouze Specificity of blebbistatin, an inhibitor of myosin II. J.Muscle Res.Cell Motil. 2004 PMID: 15548862