FR 180204

Name: FR 180204
Brand: Tocris Bioscience
SKU: 3706
Rating: 5 (2 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 3706

(2)

Citations (84)

COA / SDS

Selective ERK inhibitor

Key Product Details

Description:	Selective ERK inhibitor
Chemical Name:	5-(2-Phenyl-pyrazolo[1,5-a]pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridazin-3-ylamine
Purity:	≥98% (HPLC)
Molecular Weight:	327.34

View Full Technical Details for FR 180204

Technical Data
Citations / Reviews (2)

Biological Activity for FR 180204

FR 180204 is a selective ERK inhibitor (IC₅₀ values are 0.14 and 0.31 μM for ERK2 and ERK1 respectively). Displays 30-fold selectivity for ERK over p38α (IC₅₀ = 10 μM); displays no activity against human recombinant MEK1, MKK4, IKKα, PKCα, Src, Syc and PDGFα at concentrations less than 30 μM. Also inhibits TGFβ-induced AP-1 activation in Mv1Lu cells (IC₅₀ = 3.1 μM).

Compound Libraries for FR 180204

FR 180204 is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library and Tocriscreen Antiviral Library. Find out more about compound libraries available from Tocris.

Technical Data for FR 180204

M. Wt	327.34
Formula	C₁₈H₁₃N₇
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	865362-74-9
PubChem ID	11493598
InChi Key	XVECMUKVOMUNLE-UHFFFAOYSA-N
Smiles	NC1=NNC2=C1C=C(C3=C(C=CC=C5)N5N=C3C4=CC=CC=C4)N=N2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for FR 180204

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	32.73	100

Preparing Stock Solutions for FR 180204

The following data is based on the product molecular weight 327.34.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.05 mL	15.27 mL	30.55 mL
5 mM	0.61 mL	3.05 mL	6.11 mL
10 mM	0.31 mL	1.53 mL	3.05 mL
50 mM	0.06 mL	0.31 mL	0.61 mL

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References for FR 180204

References are publications that support the biological activity of the product.

Perrett Signaling to Extracellular Signal-regulated Kinase from ErbB1 Kinase and Protein Kinase C. J Biol Chem. 2013 PMID: 23754287
Ohori Identification of a selective ERK inhibitor and structural determination of the inhibitor-ERK2 complex. Biochem.Biophys.Res.Comm. 2005 PMID: 16139248