IHR 1

Name: IHR 1
Brand: Tocris Bioscience
SKU: 5554

Tocris Bioscience, part of Bio-Techne | Catalog # 5554

COA / SDS

Potent Smo antagonist

Key Product Details

Description:	Potent Smo antagonist
Chemical Name:	N,N'-1,4-Phenylenebis[2,5-dichlorobenzamide]
Purity:	≥99% (HPLC)
Molecular Weight:	454.13

View Full Technical Details for IHR 1

Technical Data
Citations

Biological Activity for IHR 1

IHR 1 is a potent Smo antagonist (IC₅₀ = 7.6 nM). Selectively inhibits Hedgehog signaling over Wnt and Notch signaling pathways. Blocks Smo accumulation in primary cilium in vitro.

Fluorescent Analog also available.

Technical Data for IHR 1

M. Wt	454.13
Formula	C₂₀H₁₂Cl₄N₂O₂
Storage	Store at +4°C
Purity	≥99% (HPLC)
CAS Number	548779-60-8
PubChem ID	2167042
InChi Key	VCLHHRGZKNUOAQ-UHFFFAOYSA-N
Smiles	ClC1=CC(C(NC2=CC=C(NC(C3=C(Cl)C=CC(Cl)=C3)=O)C=C2)=O)=C(Cl)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for IHR 1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	9.08	20 with gentle warming

Preparing Stock Solutions for IHR 1

The following data is based on the product molecular weight 454.13.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	11.01 mL	55.05 mL	110.10 mL
1 mM	2.20 mL	11.01 mL	22.02 mL
2 mM	1.10 mL	5.51 mL	11.01 mL
10 mM	0.22 mL	1.10 mL	2.20 mL

Molarity Calculator

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Reconstitution Calculator

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References for IHR 1

References are publications that support the biological activity of the product.

Fan The Hedgehog pathway effector smoothened exhibits signaling competency in the absence of ciliary accumulation. Chem.Biol. 2014 PMID: 25484239