Mitomycin C

Name: Mitomycin C
Brand: Tocris Bioscience
SKU: 3258
Rating: 4 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 3258

(1)

Citations (69)

COA / SDS

DNA cross-linking antitumor agent

Key Product Details

Description:	DNA cross-linking antitumor agent
Alternative Names:	Ametycine
Chemical Name:	[1aS-(1aα,8β,8aα,8bα)]-6-Amino-8-[[(aminocarbonyl)oxy]methyl]-1,1a,2,8,8a,8b-hexahydro-8a-methoxy-5-methylazirino[2',3':3,4]pyrrolo[1,2-a]indole-4,7-dione
Purity:	≥98% (HPLC)
Molecular Weight:	334.33

View Full Technical Details for Mitomycin C

Technical Data
Citations / Reviews (1)

Biological Activity for Mitomycin C

Mitomycin C is an antibiotic and antitumor agent. Covalently binds DNA forming intra- and interstrand crosslinks. Inhibits DNA synthesis. Also used for MEF/STO feeder layer preparation in stem cell culture.

Technical Data for Mitomycin C

M. Wt	334.33
Formula	C₁₅H₁₈N₄O₅
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	50-07-7
PubChem ID	5746
InChi Key	NWIBSHFKIJFRCO-WUDYKRTCSA-N
Smiles	O=C(C(N)=C1C)C2=C(N(C[C@H]4[C@@H]3N4)[C@@]3(OC)[C@@H]2COC(N)=O)C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Mitomycin C

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	0.33	1
	DMSO	33.43	100

Preparing Stock Solutions for Mitomycin C

The following data is based on the product molecular weight 334.33.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.99 mL	14.96 mL	29.91 mL
5 mM	0.60 mL	2.99 mL	5.98 mL
10 mM	0.30 mL	1.50 mL	2.99 mL
50 mM	0.06 mL	0.30 mL	0.60 mL

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Reconstitution Calculator

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References for Mitomycin C

References are publications that support the biological activity of the product.

Park Modeling and correction of structural variations in patient-derived iPSCs using CRISPR/Cas9. Nat.Protoc. 2016 PMID: 27711053
Bizanek Isolation and structure of an intrastrand cross-link adduct of mito. C and DNA. Biochemistry 1992 PMID: 1554696
Bryja Derivation of mouse embryonic stem cells. Nat.Protoc. 2006 PMID: 17487198
Tomasz Isolation and structure of a covalent cross-link adduct between mito. C and DNA. Science 1987 PMID: 3103215

Product Documents for Mitomycin C

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.

SDS

⚠ WARNING: This product can expose you to chemicals including Mitomycin C, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov