ML 299

Name: ML 299
Brand: Tocris Bioscience
SKU: 4894

Tocris Bioscience, part of Bio-Techne | Catalog # 4894

COA / SDS

Potent inhibitor of PLD1 and PLD2; CNS penetrant

Key Product Details

Description:	Potent inhibitor of PLD1 and PLD2; CNS penetrant
Chemical Name:	4-Bromo-N-[(1S)-2-[1-(3-fluorophenyl)-4-oxo-1,3,8-triazaspiro[4.5]dec-8-yl]-1-methylethyl]benzamide
Purity:	≥98% (HPLC)
Molecular Weight:	489.39

View Full Technical Details for ML 299

Technical Data
Citations

Biological Activity for ML 299

ML 299 is a selective allosteric modulator and potent inhibitor of phospholipases D1 and D2 (IC₅₀ values are 6 and 12 nM, respectively). ML 299 decreases invasive migration in glioblastoma cells and increases caspase 3/7 activity in serum-free conditions. ML 299 is non-cytotoxic, active in vivo and CNS penetrant.

Technical Data for ML 299

M. Wt	489.39
Formula	C₂₃H₂₆BrFN₄O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1426916-00-8
PubChem ID	56593054
InChi Key	RFKXAYJUFUZBMA-INIZCTEOSA-N
Smiles	O=C(N[C@@H](C)CN1CCC2(C(NCN2C3=CC(F)=CC=C3)=O)CC1)C4=CC=C(Br)C=C4

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for ML 299

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	48.94	100

Preparing Stock Solutions for ML 299

The following data is based on the product molecular weight 489.39.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.04 mL	10.22 mL	20.43 mL
5 mM	0.41 mL	2.04 mL	4.09 mL
10 mM	0.20 mL	1.02 mL	2.04 mL
50 mM	0.04 mL	0.20 mL	0.41 mL

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References for ML 299

References are publications that support the biological activity of the product.

O'Reilly Development of dual PLD1/2 and PLD2 selective inhibitors from a common 1,3,8-triazaspiro[4.5]decane core: discovery of ML298 and ML299 that decrease invasive migration in U87-MG glioblastoma cells. J.Med.Chem. 2013 PMID: 23445448