NESS 0327

Name: NESS 0327
Brand: Tocris Bioscience
SKU: 5746

Tocris Bioscience, part of Bio-Techne | Catalog # 5746

Citations (3)

COA / SDS

Ultra-high affinity and selective CB1 antagonist

Key Product Details

Description:	Ultra-high affinity and selective CB₁ antagonist
Chemical Name:	8-Chloro-1-(2,4-dichlorophenyl)-1,4,5,6-tetrahydro-N-1-piperidinyl-benzo[6,7]cyclohepta[1,2-c]pyrazole-3-carboxamide
Purity:	≥98% (HPLC)
Molecular Weight:	489.82

View Full Technical Details for NESS 0327

Technical Data
Citations

Biological Activity for NESS 0327

NESS 0327 is an ultra-high affinity and selective CB₁ receptor antagonist (K_i = 350 fM). Exhibits >60-fold selectivity for CB₁ over CB₂ receptors. Antagonizes the inhibitory effects of WIN 55,212-2 (Cat. No. 1038) on electrically evoked contractions in mouse isolated vas deferens ex vivo. Also antagonizes the antinociceptive effect of WIN 55,212-2 in tail-flick and hot plate tests in vivo.

Compound Libraries for NESS 0327

NESS 0327 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for NESS 0327

M. Wt	489.82
Formula	C₂₄H₂₃Cl₃N₄O
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	494844-07-4
PubChem ID	10435654
InChi Key	NCXBPZJQQSNIRA-UHFFFAOYSA-N
Smiles	O=C(NN1CCCCC1)C2=NN(C3=CC=C(Cl)C=C3Cl)C4=C2CCCC5=C4C=CC(Cl)=C5

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for NESS 0327

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	9.8	20

Preparing Stock Solutions for NESS 0327

The following data is based on the product molecular weight 489.82.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	10.21 mL	51.04 mL	102.08 mL
1 mM	2.04 mL	10.21 mL	20.42 mL
2 mM	1.02 mL	5.10 mL	10.21 mL
10 mM	0.20 mL	1.02 mL	2.04 mL

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References for NESS 0327

References are publications that support the biological activity of the product.

Ruiu Synthesis and characterization of NESS 0327: a novel putative antagonist of the CB₁ cannabinoid receptor. J.Pharmacol.Exp.Ther. 2003 PMID: 12663689