Pemetrexed

Name: Pemetrexed
Brand: Tocris Bioscience
SKU: 6185

Tocris Bioscience, part of Bio-Techne | Catalog # 6185

Citations (6)

COA / SDS

Thymidylate synthetase inhibitor, also inhibits dihydrofolate reductase, GARFT and AICART

Key Product Details

Description:	Thymidylate synthetase inhibitor, also inhibits dihydrofolate reductase, GARFT and AICART
Chemical Name:	N-[4-[2-(2-Amino-4,7-dihydro-4-oxo-3H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoyl]-L-glutamic acid
Purity:	≥98% (HPLC)
Molecular Weight:	427.41

View Full Technical Details for Pemetrexed

Technical Data
Citations

Biological Activity for Pemetrexed

Pemetrexed is a high affinity dihydrofolate reductase inhibitor (K_i = 7 nM). Also inhibits thymidylate synthase, AICART and glycinamide ribonucleotide formyltransferase (K_i values are 109 nM, and 3.5 μM and 9.3 μM, respectively). Indirectly activates AMPK. Inhibits proliferation of cancer cell lines in vitro.

Compound Libraries for Pemetrexed

Pemetrexed is also offered as part of the Tocriscreen FDA-Approved Drugs. Find out more about compound libraries available from Tocris.

Technical Data for Pemetrexed

M. Wt	427.41
Formula	C₂₀H₂₁N₅O₆
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	137281-23-3
PubChem ID	394493
InChi Key	WBXPDJSOTKVWSJ-UHFFFAOYSA-N
Smiles	NC1=NC(C2=C(N1)NC=C2CCC3=CC=C(C(N[C@H](C(O)=O)CCC(O)=O)=O)C=C3)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Pemetrexed

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	42.74	100

Preparing Stock Solutions for Pemetrexed

The following data is based on the product molecular weight 427.41.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.34 mL	11.70 mL	23.40 mL
5 mM	0.47 mL	2.34 mL	4.68 mL
10 mM	0.23 mL	1.17 mL	2.34 mL
50 mM	0.05 mL	0.23 mL	0.47 mL

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References for Pemetrexed

References are publications that support the biological activity of the product.

Rothbart Pemetrexed indirectly activates the metabolic kinase AMPK in human carcinomas. Cancer Res. 2010 PMID: 21159649
Shih LY231514, a pyrrolo[2,3-d]pyrimidine-based antifolate that inhibits multiple folate-requiring enzymes. Cancer Res. 1997 PMID: 9067281