Topotecan hydrochloride

Name: Topotecan hydrochloride
Brand: Tocris Bioscience
SKU: 4562

Tocris Bioscience, part of Bio-Techne | Catalog # 4562

Citations (11)

COA / SDS

DNA topoisomerase I inhibitor; Camptothecin (Cat. No. 1100) analog

Key Product Details

Description:	DNA topoisomerase I inhibitor; Camptothecin (Cat. No. 1100) analog
Alternative Names:	SK&F 104864-A
Chemical Name:	10-[(Dimethylamino)methyl]-4-ethyl-4,9-dihydroxy-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione hydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	457.91

View Full Technical Details for Topotecan hydrochloride

Technical Data
Citations

Biological Activity for Topotecan hydrochloride

Topotecan hydrochloride is a DNA topoisomerase I inhibitor; induces rapid apoptotic death in human B-lineage acute lymphoblastic leukemia (ALL) cells. Improves survival in three mouse models of poor prognosis ALL; downregulates B-Myb and MycN expression in neuroblastoma cells. Water-soluble analog of Camptothecin (Cat. No. 1100). Orally bioavailable. Topotecan disrupts the interaction between NHP2L1 and U4 RNA by binding to U4 and inhibits RNA splicing.

Technical Data for Topotecan hydrochloride

M. Wt	457.91
Formula	C₂₃H₂₃N₃O₅.HCl
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	119413-54-6
PubChem ID	60699
InChi Key	DGHHQBMTXTWTJV-BQAIUKQQSA-N
Smiles	OC1=CC=C(N=C(C(N3C4)=CC([C@](CC)(O)C(OC5)=O)=C5C3=O)C4=C2)C2=C1CN(C)C.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Topotecan hydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	45.79	100
	DMSO	45.79	100

Preparing Stock Solutions for Topotecan hydrochloride

The following data is based on the product molecular weight 457.91.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.18 mL	10.92 mL	21.84 mL
5 mM	0.44 mL	2.18 mL	4.37 mL
10 mM	0.22 mL	1.09 mL	2.18 mL
50 mM	0.04 mL	0.22 mL	0.44 mL

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References for Topotecan hydrochloride

References are publications that support the biological activity of the product.

Sottile A chemical screen identifies the chemotherapeutic drug topo. as a specific inhibitor of the B-MYB/MYCN axis in neuroblastoma. Oncotarget. 2012 PMID: 22619121
Schellens Bioavailability and pharmacokinetics of oral topotecan: a new topoisomerase I inhibitor. Br.J.Cancer 1996 PMID: 8630291
Uckun In vitro and in vivo activity of topo. against human B-lineage acute lymphoblastic leukemia cells. Blood 1995 PMID: 7742543