Fluorescent probes target specific cellular and sub-cellular components, or they may selectively target a single biomolecule. Alternatively, they might provide a functional read-out, such as live versus dead cell staining. They enable researchers to detect particular components of complex biomolecular assemblies, such as microtubules, with high sensitivity and selectivity allowing the exploration of cell structure and function. Bio-Techne, through the Tocris brand, offers a wide selection of fluorescent probes, covering organelle probes and cell viability stains.
MitoBrilliant™ Dyes
MitoBrilliant are next-generation fluorescent stains for the localization and tracking of mitochondria in both live and fixed cells. Our range harnesses Janelia Fluor® dye technology, conferring some of the properties that make these widely used dyes, into mitochondrial stains. The MitoBrilliant Live dyes accumulate in the mitochondria of live cells. Two dyes are available: MitoBrilliant™ Live 646 and MitoBrilliant™ Live 549. A universal stain for mitochondria in both live and fixed cells is also available: MitoBrilliant™ 646.
MitoBrilliant™ 646
MitoBrilliant™ 646
- Suitable for Live and Fixed Cell Applications Including for Staining Pre-Fixed Cells
- Improved Performance and Staining Fidelity Post-Fixation
Product Name |
Core Dye Structure |
Abs/Em (nm) |
Δψm Dependent |
Live/Fixed Cell Use? |
Stains Pre-Fixed Cells? |
Image Without Wash Step? |
Demonstrated Applications |
|
MitoBrilliant™ 646 Cat. No. 7700 |
Janelia Fluor® technology |
655/668 |
No* |
Suitable for live and fixed cell work |
Yes |
Yes, but replacing media recommended |
Fixed-cell imaging, Live-cell imaging Flow cytometry, IHC/ICC, Super-resolution, Microscopy – STED, High-content screening |
|
MitoBrilliant™ Live 646 Cat. No. 7417 |
Janelia Fluor® technology |
648/662 |
Yes |
Live-cell work only |
No |
Yes, but replacing media recommended |
Live-cell imaging, Flow cytometry, High-content screening |
|
MitoBrilliant™ Live 549 Cat. No. 7693 |
Janelia Fluor® technology |
550/568 |
Yes |
Live-cell work only |
No |
Yes, but replacing media recommended |
Live-cell imaging, Flow cytometry, High-content screening |
* In live-cell staining, Δψm drives initial recruitment of the dye into mitochondria. After staining, localization of the dyes becomes insensitive to Δψm changes. In pre-fixed cell staining, MitoBrilliant™ 646 can localize and stay in mitochondria without Δψm.
Fluorescent Microtubule Probes
Fluorescent Cell Metabolism Probes
Flow Cytometry
Bio-Techne has a large catalog of antibodies, reagents and tools to streamline your flow cytometry experiments.
Fluorescent Dyes
Bio-Techne through the Tocris brand, offers a wide range of fluorescent dyes including bright and photostable Janelia fluor dyes.
FluorokinesTM: Fluorescently Labeled Proteins
Easily measure transduction efficiency of CAR T or CAR NK cell preparations or determine B cell specificity after COVID-19 infection or vaccination.
Spectra Viewer
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
MitoBrilliant Guide
This product guide provides a background, protocols and data from use in different research applications for our Mitobrilliant fluorescent mitochondrial probes.
Fluorescent Dyes and Probes Brochure
Our brochure showcases our range and gives background on the use of Fluorescent Dyes and Probes.
