Product Overview
The RNAscope™ Multiomic LS Assay enables highly sensitive and specific detection of both RNA and proteins in the same tissue section for a true multiomic spatial assay. Proven class-leading single-molecule RNAscope technology is leveraged to enable simultaneous detection of up to 6 total proteins and RNA targets with single cell resolution.
This assay is based on the same technology as the RNAscope Multiplex v2 assay and is compatible with your existing probes and workflows. With both protease and a new completely protease-free workflow, the kit provides significantly upgraded capabilities and benefits.
The RNAscope™ Multiomic LS Assay is designed as an automated assay for the Leica Biosystems’ BOND RX Research Advanced Staining System.
Figure 1 (right): Human cervical cancer tissue. Proteins detected: CD4 (yellow), CD8 (white), PanCK (pink), & FoxP3 (green). RNAs detected: GZMB (orange) & IFNG (red).
Optimized Protease-Free Multiomic Workflow
Optimized Protease-Free Multiomic Workflow
- Preserves antigen integrity for wide antibody compatibility.
- Use your own primary and secondary antibodies in sequential co-detection workflows. Follow RNAscope detection with your usual IHC.
- Maintains tissue morphology for accurate spatial analysis.
- Robust RNAscope Tyramide Signal Amplification (TSA) chemistry for both RNA and protein detection
Fast and Flexible Assay Design
Fast and Flexible Assay Design
- Up to 6 total plex.
- Detect any RNA transcripts. Any gene. Any species.
- Select from pre-qualified RNAscope antibodies to accelerate multiomic assay design at higher plex.
Gold-standard RNAscope Detection
Gold-standard RNAscope Detection
- Proven performance for FFPE samples and high to low expressing target genes.
- Backed by over 11K publications in peer-reviewed journals
- Over 50K probes visualized
- Compatible with downstream H&E staining
Technology Overview
The RNAscope Multiomic LS assay includes two key innovations to enable the best RNA in situ hybridization results AND the best protein detection, on the same slide.
Protease-free Workflow
Expanded Protein Detection: The new RNAscope Protease-Free workflow now empowers you to use any IHC-validated antibody to co-detect proteins and RNAs on the same tissue section.
Preserved Tissue Morphology: The protease-free approach preserves tissue morphology, ensuring that your results are both precise for analysis and visually stunning.
Flexible Assay Design: Kit includes both Protease and Protease-Free reagents, so you can choose from either pretreatment options.
Ease of implementation: The Protease-Free workflow with PretreatPro seamlessly integrates with your existing RNAscope protocols.
Learn more about the benefits of Protease-free pretreatment.
RNAscope Technology, Brought to Protein Detection
RNA Detection
RNA Detection
RNAscope Chemistry
Visualize RNAs with RNAscope Multiplex v2 detection technology. RNA-specific target probes using proprietary double ZZ design are amplified using RNAscope technology followed by fluorescent TSA detection.
Protein Detection
Protein Detection
Visualize proteins using ready-to-use pre-conjugated primary antibodies or your own unconjugated primary antibodies together with available pre-qualified secondary antibodies. Antibodies are detected using RNAscope amplification technology followed by fluorescent TSA detection.
Immunology and Inflammation
Immunology and Inflammation
- Assess cytokine profiles and identify their cellular source
- Visualize gene signatures with cell-specific markers to assess activation states of immune cell
Tumor-Associated Macrophages Visualized Using the Multiomic LS Assay
- Assess cytokine profiles and identify their cellular source
- Visualize gene signatures with cell-specific markers to assess activation states of immune cells
Image: Human breast cancer tissue stained for 2 protein targets (CD68 and CD163) and 4 RNA targets (IL10, IL-1B, TNFα, and CXCL10).
Fast & Flexible Assay Design
The RNAscope Multiomic protease-free workflow enables easy and robust integration with sequential RNA and protein detection workflows.
For increased multiomic capability, the assay can be used with primary antibodies selected from a pre-qualified panel targeting the most common cell markers. These panels ensure sensitivity and specificity and that the antibodies work together in the assay, minimizing optimization time and effort. Select up to four protein targets from the panel and combine with your own primary antibodies and available conjugated secondaries for added protein detection capability.
Finally, add probes to any gene to quickly build your own custom assay.
Multiomic detection with RNAscope antibodies. Easy as 1, 2, 3!
Fluorophore Selection
The RNAscope Multiomic LS assay supports detection of up to 6 targets simultaneously. The following are the recommended TSA fluorophores to maximize sensitivity and minimize spectral overlap. If using other TSA fluorophores, select the best combination for your imaging system.
| Bio-Techne TSA Fluorophore Kits | Catalog # | 3-plex |
|---|---|---|
| TSA Vivid™ Fluorophore Kit 520 | 323271 | ✔ |
| TSA Vivid™ Fluorophore Kit 570 | 323272 | ✔ |
| TSA Vivid™ Fluorophore Kit 650 | 323273 | ✔ |
| Opal TSA Fluorophores | Akoya Biosciences Catalog # | 3-plex | 4-plex | 5- or 6-plex |
|---|---|---|---|---|
| Opal 480 Reagent Pack | FP1500001KT | ✔ | ||
| Opal 520 Reagent Pack | FP1487001KT | ✔ | ✔ | ✔ |
| Opal 570 Reagent Pack | FP1488001KT | ✔ | ✔ | ✔ |
| Opal 620 Reagent Pack | FP1495001KT | ✔ | ||
| Opal 690 Reagent Pack | FP1497001K | ✔ | ✔ | ✔ |
| Opal Polaris 780 Reagent Pack Antibody Diluent/Block (needed when using Opal Polaris 780) | ARO1001EA | ✔ | ✔ |
NOTE: For 5-plex assay, select any 5 of the recommended fluorophores.
What Should I Order?
| Assay Components | Catalog # | Number of Assays |
|---|---|---|
| RNAscope™ Multiomic CORE & C1 - C3 Channel Kit | 323155 323460 | 60 20 |
| RNAscope™ Multiomic CORE & C1 - C6 Channel Kit | 323175 323465 | 60 20 |
| RNAscope™ Multiomic C4 Channel Reagents | 322950 323445 | 60 20 |
| RNAscope™ Multiomic C5 Channel Reagents | 322955 323450 | 60 20 |
| RNAscope™ Multiomic C6 Channel Reagents | 322960 323455 | 60 20 |
| Human TIL Panel Primary Antibodies | Catalog # | Number of Assays |
|---|---|---|
| RNAscope™ Ab Hs CD4-C3 | 322949 | 20 |
| RNAscope™ Ab Hs-CD8-C4 | 322951 | 20 |
| RNAscope™ Ab-Hs-PanCK-C5 | 322952 | 20 |
| RNAscope™ Ab-Hs FoxP3-C6 | 322953 | 20 |
RNAscope™ Multiomic Human TIL Antibody Panel Pack includes CD4, CD8, PanCK and FoxP3 antibodies | 323185 | 20 |
Neuro Panel Primary Antibodies Cross reacts against mouse, human, and non-human primate targets. | Catalog # | Number of Assays |
|---|---|---|
| RNAscope™ Ab NeuN-C3 (Coming Soon) | AB0018-C3 | 20 |
| RNAscope™ Ab GFAP-C4 (Coming Soon) | AB0028-C4 | 20 |
| RNAscope™ Ab IBA-1-C5 (Coming Soon) | AB0038-C5 | 20 |
RNAscope™ Multiomic Neuronal Antibody Panel Pack includes NeuN, GFAP and IBA-1 antibodies | 321780 | 20 |
| RNAscope Secondary Antibodies | Catalog # | Number of Assays |
|---|---|---|
| RNAscope™ anti-rabbit-C1 | 322954 | 60 |
| RNAscope™ anti-mouse-C2 | 322956 | 60 |
| Control Probes | Catalog # | Number of Assays |
|---|---|---|
RNAscope™ Multiomic LS 6-plex Positive Control Probe-Hs (RTU mixture of 6 probes targeting human housekeeping genes in each of 6 separate channels: Hs-POLR2A-C1, Hs-PPIB-C2, Hs-UBC-C3, Hs-HPRT1-C4, Hs-ACTB-C5, Hs-TUBB-C6.) | 323198 | 30 |
| Hs-Polr2A | 310458-C1 | 30 |
| Hs-PPIB-C2 | 313908-C2 | 30 |
| Hs-UBC-C3 | 312028-C3 | 30 |
| Hs-HPRT1-C4 | 310348-C4 | 30 |
| Hs-ACTB-C5 | 310148-C5 | 30 |
| Hs-TUBB-C6 | 588798-C6 | 30 |
| RNAscope™ Multiomic LS 6-plex Negative Control Probe | 323208 | 30 |
| RNAscope™ Multiomic LS 6-plex Positive Control Probe-Mn (RTU mixture of 6 probes targeting mouse housekeeping genes in each of 6 separate channels. Mm-POLR2A-C1, Mm-PPIB-C2, Mm-UBC-C3, Mm-HPRT1-C4, Mm-ACTB-C5, Mm-Sdha-C6) | 323988 | 30 |
RNAscope™ Multiomic LS Assay Brochure
Please Note: For ordering checklists and user manual please see the documents section on each individual product page to download.
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