Product Overview
The RNAscope™ Multiomic LS Assay enables highly sensitive and specific detection of both RNA and proteins in the same tissue section for a true multiomic spatial assay. Proven class-leading single-molecule RNAscope technology is leveraged to enable simultaneous detection of up to 6 total proteins and RNA targets with single cell resolution.
This assay is based on the same technology as the RNAscope Multiplex v2 assay and is compatible with your existing probes and workflows. With both protease and a new completely protease-free workflow, the kit provides significantly upgraded capabilities and benefits.
The RNAscope™ Multiomic LS Assay is designed as an automated assay for the Leica Biosystems’ BOND RX Research Advanced Staining System.
Figure 1 (right): Human cervical cancer tissue. Proteins detected: CD4 (yellow), CD8 (white), PanCK (pink), & FoxP3 (green). RNAs detected: GZMB (orange) & IFNG (red).
Choose Your Protease-Free Multiomic Workflow
Choose Your Protease-Free Multiomic Workflow
- Protease-free preserves antigen integrity for wide antibody compatibility and maintenance of tissue morphology.
Use a sequential co-detection workflow by performing RNAscope detection and following with your usual IHC.
Or
- Use the full RNAscope Multiomic workflow for more protein plex and minimal optimization by selecting from a pre-qualified RNAscope antibodies.
Note: Kit also includes protease to support your ongoing projects.
Fast and Flexible Assay Design
Fast and Flexible Assay Design
- Up to 6 total plex.
- RNAscope probes let you detect any RNA transcripts. Any gene. Any species.
- Accelerate multiomic assay design at higher plex by selecting from RNAscope antibodies. Pre-qualified RNAscope primary and secondary antibodies minimize optimization time.
Gold-Standard RNAscope Detection
Gold-Standard RNAscope Detection
- Proven performance for FFPE samples and high to low expressing target genes.
- Backed by over 12K publications in peer-reviewed journals
- Over 70K probes visualized
- Compatible with downstream H&E staining
Protease-Free Workflow Flexibility
RNA Only
- For unparalleled sensitivity and specificity.
- Detect up to 6 RNA targets at the same time.
- Protease-free reagents maintain tissue morphology for precise spatial analysis.
- Kit includes both protease-free and protease reagents for maximum compatibility.
Sequential Co-Detection
- Easily perform RNA and protein detection sequentially.
- First, perform RNAscope detection with protease-free reagents to preserve antigen integrity.
- Then simply follow with standard IHC or IF detection using your own primary and secondary antibodies.
Full Multiomic Workflow
- For increased protein plex, include RNAscope antibodies for a complete RNA and protein assay.
- Select from pre-qualified RNAscope primary antibodies against popular targets.
- For even more flexibility, use your own primaries with RNAscope secondary antibodies AND RNAscope primary antibodies.
- For a total of 6 RNA and protein targets.
Tumor-Associated Macrophages Visualized Using the Multiomic LS Assay
- Assess cytokine profiles and identify their cellular source
- Visualize gene signatures with cell-specific markers to assess activation states of immune cells
Image: Human breast cancer tissue stained for 2 protein targets (CD68 and CD163) and 4 RNA targets (IL10, IL-1B, TNFα, and CXCL10).
Immunology and Inflammation
Immunology and Inflammation
- Assess cytokine profiles and identify their cellular source
- Visualize gene signatures with cell-specific markers to assess activation states of immune cell
Full Multiomics with RNAscope Antibodies. Easy as 1, 2, 3!
The RNAscope Multiomic protease-free workflow enables easy and robust integration with sequential RNA and protein detection workflows.
For increased multiomic capability, the assay can be used with primary antibodies selected from a pre-qualified panel targeting the most common cell markers. These panels ensure sensitivity and specificity and that the antibodies work together in the assay, minimizing optimization time and effort. Select up to four protein targets from the panel and combine with your own primary antibodies and available conjugated secondaries for added protein detection capability.
Finally, add probes to any gene to quickly build your own custom assay.
Fluorophore Selection
The RNAscope Multiomic LS assay supports detection of up to 6 targets simultaneously. The following are the recommended TSA fluorophores to maximize sensitivity and minimize spectral overlap. If using other TSA fluorophores, select the best combination for your imaging system.
Bio-Techne TSA Fluorophore Kits | Catalog # | 3-plex |
|---|---|---|
| TSA Vivid™ Fluorophore Kit 520 | 323271 | ✔ |
| TSA Vivid™ Fluorophore Kit 570 | 323272 | ✔ |
| TSA Vivid™ Fluorophore Kit 650 | 323273 | ✔ |
Opal TSA Fluorophores | Akoya Biosciences Catalog # | 3-plex | 4-plex | 5- or 6-plex |
|---|---|---|---|---|
| Opal 480 Reagent Pack | FP1500001KT | ✔ | ||
| Opal 520 Reagent Pack | FP1487001KT | ✔ | ✔ | ✔ |
| Opal 570 Reagent Pack | FP1488001KT | ✔ | ✔ | ✔ |
| Opal 620 Reagent Pack | FP1495001KT | ✔ | ||
| Opal 690 Reagent Pack | FP1497001K | ✔ | ✔ | ✔ |
| Opal Polaris 780 Reagent Pack Antibody Diluent/Block (needed when using Opal Polaris 780) | ARO1001EA | ✔ | ✔ |
NOTE: For 5-plex assay, select any 5 of the recommended fluorophores.
What Should I Order?
Assay Components | Catalog # | Number of Assays |
|---|---|---|
| RNAscope™ Multiomic CORE & C1 - C3 Channel Kit | 323155 323460 | 60 20 |
| RNAscope™ Multiomic CORE & C1 - C6 Channel Kit | 323175 323465 | 60 20 |
| RNAscope™ Multiomic C4 Channel Reagents | 322950 323445 | 60 20 |
| RNAscope™ Multiomic C5 Channel Reagents | 322955 323450 | 60 20 |
| RNAscope™ Multiomic C6 Channel Reagents | 322960 323455 | 60 20 |
Human TIL Panel Primary Antibodies | Catalog # | Number of Assays |
|---|---|---|
| RNAscope™ Ab Hs CD4-C3 | 322949 | 20 |
| RNAscope™ Ab Hs-CD8-C4 | 322951 | 20 |
| RNAscope™ Ab-Hs-PanCK-C5 | 322952 | 20 |
| RNAscope™ Ab-Hs FoxP3-C6 | 322953 | 20 |
RNAscope™ Multiomic Human TIL Antibody Panel Pack includes CD4, CD8, PanCK and FoxP3 antibodies | 323185 | 20 |
Neuro Panel Primary AntibodiesCross reacts against mouse, human, and non-human primate targets. | Catalog # | Number of Assays |
|---|---|---|
| RNAscope™ Ab NeuN-C3 (Coming Soon) | AB0018-C3 | 20 |
| RNAscope™ Ab GFAP-C4 (Coming Soon) | AB0028-C4 | 20 |
| RNAscope™ Ab IBA-1-C5 (Coming Soon) | AB0038-C5 | 20 |
RNAscope™ Multiomic Neuronal Antibody Panel Pack includes NeuN, GFAP and IBA-1 antibodies | 321780 | 20 |
RNAscope Secondary Antibodies | Catalog # | Number of Assays |
|---|---|---|
| RNAscope™ anti-rabbit-C1 | 322954 | 60 |
| RNAscope™ anti-mouse-C2 | 322956 | 60 |
Control Probes | Catalog # | Number of Assays |
|---|---|---|
RNAscope™ Multiomic LS 6-plex Positive Control Probe-Hs (RTU mixture of 6 probes targeting human housekeeping genes in each of 6 separate channels: Hs-POLR2A-C1, Hs-PPIB-C2, Hs-UBC-C3, Hs-HPRT1-C4, Hs-ACTB-C5, Hs-TUBB-C6.) | 323198 | 30 |
| Hs-Polr2A | 310458-C1 | 30 |
| Hs-PPIB-C2 | 313908-C2 | 30 |
| Hs-UBC-C3 | 312028-C3 | 30 |
| Hs-HPRT1-C4 | 310348-C4 | 30 |
| Hs-ACTB-C5 | 310148-C5 | 30 |
| Hs-TUBB-C6 | 588798-C6 | 30 |
| RNAscope™ Multiomic LS 6-plex Negative Control Probe | 323208 | 30 |
| RNAscope™ Multiomic LS 6-plex Positive Control Probe-Mn (RTU mixture of 6 probes targeting mouse housekeeping genes in each of 6 separate channels. Mm-POLR2A-C1, Mm-PPIB-C2, Mm-UBC-C3, Mm-HPRT1-C4, Mm-ACTB-C5, Mm-Sdha-C6) | 323988 | 30 |
RNAscope™ Multiomic LS Assay Brochure
Please Note: For ordering checklists and user manual please see the documents section on each individual product page to download.
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