Spatial analysis of tumor-infiltrating immune cells with highly specific RNAscope™ RNA-protein Co-detection assays

Interrogating complex tumor microenvironment requires a multiomics approach that can provide high level of sensitivity and specificity. Identifying immune cell subsets within the tumor can be vital for predicting response and determining therapeutic efficacy. Here, we demonstrate a newly developed integrated ISH and IHC/IF (immunohistochemistry/ immunofluorescence) workflow compatible with manual and automated platforms that can substantially improve RNA-protein co-detection. We show that this new RNA-protein Co-detection workflow improves IHC antibody compatibility with the RNAscope ISH technology creating an extremely powerful multiomic solution to elucidate cellular heterogeneity, identify novel cell populations while retaining spatial information with morphological context.