Superplex - More than a Multiplex Immunoassay

Superplex assays on Jess™ go far beyond the traditional multiplex immunoassay on Western blot and other protein analysis systems, so we named it something else. Jess automates the protein separation and immunodetection of traditional Western blotting, eliminating tedious and error-prone steps. With both multiplex fluorescent immunoassay and chemiluminescent detection capabilities combined with in-capillary protein normalization, you can efficiently develop an assay to get more data points out of one sample.

What is Superplex?

Multiplex Up To Three Channels

Superplexing using Jess’s infrared (IR), near-infrared (NIR) and chemiluminescence channels helps you identify and differentiate relative protein expression between samples while built-in Protein Normalization gives you the confidence you need in your results all in one shot. Your sample, antibodies, and Protein Normalization and other reagents are loaded automatically, and all steps of a Superplex multiplex immunoassay take place in a single capillary. Jess first detects the total amount of protein present in your sample with the Protein Normalization Module, and then probes for your targets of interest in order of multiplex fluorescent immunoassay first, followed by chemiluminescence detection.

• Run chemiluminescence and fluorescence multiplex immunoassays on the same sample at the same time
• Look at multiple targets and parameters simultaneously
• Get info on protein abundance, isoform and size all at once
• Throw in total protein without giving up a detection channel
• Detect high and low abundance proteins in the same sample, even when they have identical molecular weights
• Save time—your multiplexed, normalized, quantitative, size-based data will be ready in 3.5 hours
• Conserve precious samples—Jess only needs 3 µL/sample

Superplexing is Simple

All you have to do is load your samples, antibodies and reagents into a plate, insert the plate and capillary cartridge and press Start! Then enjoy 3 hours of handsfree run time to focus on your science, product development, or project deadline. When Jess is done, come back to fully analyzed and quantitative results! Compass for Simple Western automatically analyzes the data when the run is complete, including normalizing target data.

More Western Blot Data in Less Time

When you Superplex with Jess, a multiplex immunoassay platform, just 3 µL of sample gives you at least 4 data points. Not to mention the time you save with your Western blots by doing four things at once, and that it only takes 3.5 hours to do them. But those aren't the only pros of Superplexing. Because each of those data points gets captured in the same capillary, you're not spending time comparing, infering and interpreting data across runs, samples, or lanes either. That means you get to make more informed, confident conclusions about your Western blot test results.

Figure caption: Protein normalization reveals comparable p-Akt signals across increasing concentrations of Jurkat + Calyculin A lysate. Comparative data showing p-Akt protein expression detected on Jess’s chemiluminescence channel and total Akt detected by the NIR channel as a measure of raw peak area shown in orange bars and separate-channel normalization for the normalized peak area shown in blue bars. As expected, normalization of the raw data results in similar peak area across all three lysate concentrations. Values plotted are mean protein peak areas for samples run in triplicate.