14-3-3 gamma [ac Val2] Antibody (KC21) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-406

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # KC21

Format

BSA Free

Concentration

1.0 mg/ml

View all 14-3-3 gamma Antibodies »

Product Specifications

Immunogen

N-terminal fragment of human 14-3-3 gamma where the N-terminal Val was acetylated [UniProt# P61981]

Modification

ac Val2

Localization

Cytoplasmic

Specificity

This antibody is specific for the naturally occurring human form of 14-3-3 gamma, where the N-terminal Met is removed, resulting in an acetylated N-terminal Val. Unprocessed (non-modified) 14-3-3 gamma is not recognized by this antibody.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 14-3-3 gamma [ac Val2] Antibody (KC21) - BSA Free

Western Blot: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - (1) HeLa, (2) PC12 and (3) NIH-3T3 whole cell lysates were separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was probed with Anti-14-3-3 gamma [Ac Val2] antibody at 2 ug/ml for 1 hour, followed by incubation a 1:10,000 dilution of anti-rabbit HRP. The protein was detected at approximately 28 kDa after incubation with SuperSignal West Pico Chemiluminescent substrate.
Immunocytochemistry/Immunofluorescence: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - 14-3-3 gamma [ac Val2] antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Western Blot: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - 14-3-3 gamma detected in lysates using NB100-406. Lane 1: HeLa cell lysates, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).
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Applications for 14-3-3 gamma [ac Val2] Antibody (KC21) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Simple Western

1:12.5

Western Blot

1:1000-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 14-3-3 gamma

14-3-3 proteins are highly conserved and ubiquitously expressed proteins present as multigene families in most of the living organisms wherein they play a key role of bringing signal transduction to completion, and implicates in regulating several cellular processes including apoptosis, cell-cycle checkpoints and nutrient-sensing signaling pathways. 14-3-3 proteins have several isoforms which include -beta, -gamma, -epsilon, -sigma, -zeta, -tau, and -eta. 14-3-3 proteins can form homo- or heterodimers which interacts with a wide variety of proteins such as transcription factors, enzymes/kinases, cytoskeletal proteins, phosphatases, and other signaling molecules. 14-3-3 interactions with its targets occurs primarily via phospho-Ser/Thr motif, however, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions have been shown. 14-3-3 binding masks specific sequences of target proteins, thereby altering their localization, phosphorylation state, stability, and molecular interactions, and also induces target protein conformational changes which results in altered target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in developmental and disease signaling which underlies their different functions despite sequence similarities.

Alternate Names

1433 gamma, YWHAG

Entrez Gene IDs

7532 (Human)

Gene Symbol

YWHAG

UniProt

P61981

Additional 14-3-3 gamma Products

Product Documents for 14-3-3 gamma [ac Val2] Antibody (KC21) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for 14-3-3 gamma [ac Val2] Antibody (KC21) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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