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14-3-3 gamma [ac Val2] Antibody (KC21) [DyLight 755]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-406IR

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Label

DyLight 755 (Excitation = 754 nm, Emission = 776 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # KC21

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

N-terminal fragment of human 14-3-3 gamma where the N-terminal Val was acetylated [UniProt# P61981]

Modification

ac Val2

Localization

Cytoplasmic

Specificity

This antibody is specific for the naturally occurring human form of 14-3-3 gamma, where the N-terminal Met is removed, resulting in an acetylated N-terminal Val. Unprocessed (non-modified) 14-3-3 gamma is not recognized by this antibody.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for 14-3-3 gamma [ac Val2] Antibody (KC21) [DyLight 755]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: 14-3-3 gamma

14-3-3 protein gamma is a member of the 14-3-3 family of proteins which help mediate signal transduction. Specifically, 14-3-3 gamma is an adapter protein responsible for bringing signal transduction to completion. It therefore plays an important role in regulating many fundamental cellular processes, such as apoptosis and cell-cycle checkpoints.

Signal-induced phosphorylation has the ability to change protein function, however phosphorylation is not always enough to change a protein's function. 14-3-3 gamma has the ability to bind a large number of target proteins thereby affecting the proteins' activity and/or function.

14-3-3 gamma is highly expressed in brain, heart and skeletal muscle, where it is induced by growth factors in human vascular smooth muscle cells. This suggests that 14-3-3 gamma may play a role in muscle tissue function.

Alternate Names

1433 gamma, YWHAG

Gene Symbol

YWHAG

Additional 14-3-3 gamma Products

Product Documents for 14-3-3 gamma [ac Val2] Antibody (KC21) [DyLight 755]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 14-3-3 gamma [ac Val2] Antibody (KC21) [DyLight 755]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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