14-3-3 gamma [ac Val2] Antibody (KC21) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-406CL1
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG2A Clone # KC21
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
N-terminal fragment of human 14-3-3 gamma where the N-terminal Val was acetylated [UniProt# P61981]
Modification
ac Val2
Specificity
This antibody is specific for the naturally occurring human form of 14-3-3 gamma, where the N-terminal Met is removed, resulting in an acetylated N-terminal Val. Unprocessed (non-modified) 14-3-3 gamma is not recognized by this antibody.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for 14-3-3 gamma [ac Val2] Antibody (KC21) [CoraFluor™ 1]
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Simple Western
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: 14-3-3 gamma
Signal-induced phosphorylation has the ability to change protein function, however phosphorylation is not always enough to change a protein's function. 14-3-3 gamma has the ability to bind a large number of target proteins thereby affecting the proteins' activity and/or function.
14-3-3 gamma is highly expressed in brain, heart and skeletal muscle, where it is induced by growth factors in human vascular smooth muscle cells. This suggests that 14-3-3 gamma may play a role in muscle tissue function.
Alternate Names
1433 gamma, YWHAG
Gene Symbol
YWHAG
Additional 14-3-3 gamma Products
Product Documents for 14-3-3 gamma [ac Val2] Antibody (KC21) [CoraFluor™ 1]
Product Specific Notices for 14-3-3 gamma [ac Val2] Antibody (KC21) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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