Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # ALDH1A1/1381

Concentration

0.2 mg/ml

View all Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibodies »

Product Summary for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381)

Immunogen

Recombinant fragment (around aa 315-434) of human Aldehyde Dehydrogenase 1-A1/ALDH1A1 protein (exact sequence is proprietary). (Uniprot: P00352)

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54422)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381)

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248]

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248]

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248] - Western Blot Analysis of human Liver tissue lysate using Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381).
Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248]

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248]

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248] - Formalin-fixed, paraffin-embedded Human Testicular Carcinoma stained with ALDH1A1 Monoclonal Antibody (ALDH1A1/1381).
Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248]

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248]

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248] - Western Blot of K562 and Lung lysate Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381).
View 1 more images

Applications for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Aldehyde Dehydrogenase 1-A1/ALDH1A1

Usual human livers contain two major ALDH isozymes, i.e., cytosolic ALDH1 and mitochondrial ALDH2(1). Human red cell aldehyde dehydrogenase (ALDH) resembles the liver cytosolic isozyme in numerous physicochemical properties. Thus, the erythrocyte enzyme, by virtue of its chemical and structural identity with the liver cytosolic enzyme, may serve as a suitable peripheral enzyme model to understand the cause and mechanism of alcohol abuse-related changes in liver cytosolic ALDH that has been found to be reduced in alcoholics (2). ALDH1A1 is known to catalyze the oxidation of retinaldehyde to retinoic acid. Overexpression of human ALDH1A1 in HEK cells led to a more than 20-fold increase in 3-deoxyglucosone dehydrogenase activity (3).

Alternate Names

ALDC, Aldehyde Dehydrogenase 1A1, ALDH-E1, ALDH1A1, PUMB1, RALDH1

Gene Symbol

ALDH1A1

UniProt

P00352

Additional Aldehyde Dehydrogenase 1-A1/ALDH1A1 Products

Product Documents for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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