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Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2002

Novus Biologicals, part of Bio-Techne
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NB100-2002-0.1mg

Key Product Details

Species Reactivity

Validated:

Yeast

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Biotin

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

Product Summary for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Biotin]

Immunogen

Aldehyde Dehydrogenase 1-A1/ALDH1A1 1-A1/ALDH1A1 [Yeast] (Uniprot: P47771)

Reactivity Notes

Cross reactivity against Aldehyde Dehydrogenase 1-A1/ALDH1A1 from other tissues and species may occur but have not been specifically determined.

Specificity

Cross reactivity against Aldehyde Dehydrogenase 1-A1/ALDH1A1 from other tissues and species may occur but have not been specifically determined.

Marker

Astrocyte Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Aldehyde Dehydrogenase [Yeast]

Applications for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Biotin]

Application
Recommended Usage

ELISA

1:1000-1:10000

Immunoprecipitation

1:100

Western Blot

1:500-1:5000
Application Notes
This product has been tested by ELISA and Western blot. This product is assayed against 1.0 ug of Aldehyde Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: Aldehyde Dehydrogenase 1-A1/ALDH1A1

ADH (alcohol dehydrogenase) family of proteins metabolize a variety of substances such as ethanol, retinal, other aliphatic alcohol, hydroxysteroids, and lipid peroxidation products. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohol to ketones or aldehydes. ADH play a major role in ethanol metabolism.

Alternate Names

ALDC, Aldehyde Dehydrogenase 1A1, ALDH-E1, ALDH1A1, PUMB1, RALDH1

Gene Symbol

ALDH1A1

UniProt

Additional Aldehyde Dehydrogenase 1-A1/ALDH1A1 Products

Product Documents for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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