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beta 2-Microglobulin Antibody (B2M-01) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-60015

Novus Biologicals, part of Bio-Techne
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NB110-60015

Key Product Details

Species Reactivity

Validated:

Human, Bovine (Negative), Canine (Negative), Chicken (Negative), Mouse (Negative), Rabbit (Negative)

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # B2M-01

Concentration

1.0 mg/ml

Product Summary for beta 2-Microglobulin Antibody (B2M-01) [FITC]

Immunogen

Purified human beta 2 Microglobulin (NM_004048.2).

Reactivity Notes

Weak positive staining was observed in peripheral blood lymphocytes of the monkey Cercopithecus aethiops.

Specificity

The antibody B2M-01 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta 2-Microglobulin Antibody (B2M-01) [FITC]

Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [FITC] [NB110-60015] - Staining of beta2-microglobulin in human peripheral blood with anti-beta 2-microglobulin (B2M-01) FITC.

Applications for beta 2-Microglobulin Antibody (B2M-01) [FITC]

Application
Recommended Usage

Flow Cytometry

1 ug/ml
Application Notes
Purity is > 95% (by SDS-PAGE)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

PBS (pH 7.4)

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: beta 2-Microglobulin

Beta 2 Microglobulin non covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.

Alternate Names

B2M, beta 2Microglobulin

Gene Symbol

B2M

Additional beta 2-Microglobulin Products

Product Documents for beta 2-Microglobulin Antibody (B2M-01) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for beta 2-Microglobulin Antibody (B2M-01) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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