beta 2-Microglobulin Antibody (B2M-01) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-317

Novus Biologicals, part of Bio-Techne
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NB500-317
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Key Product Details

Species Reactivity

Validated:

Human, Bovine (Negative), Canine (Negative), Chicken (Negative), Mouse (Negative), Rabbit (Negative)

Cited:

Human, Bovine

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Radioimmunoassay, Western Blot

Cited:

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # B2M-01

Format

BSA Free

Concentration

1 mg/ml

View all beta 2-Microglobulin Antibodies »

Product Specifications

Immunogen

Purified human beta 2 Microglobulin (NM_004048.2).

Reactivity Notes

Weak positive staining was observed in peripheral blood lymphocytes of the monkey Cercopithecus aethiops.

Specificity

The antibody B2M-01 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta 2-Microglobulin Antibody (B2M-01) - BSA Free

Western Blot: beta 2-Microglobulin Antibody (B2M-01)BSA Free [NB500-317]

Western Blot: beta 2-Microglobulin Antibody (B2M-01)BSA Free [NB500-317]

Western Blot: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Analysis using the FITC conjugate of NB500-317. Detection (non-reducing conditions) of whole cell lysate of various cell lines Lane 1: RAJI human Burkitt lymphoma cell line Lane 2: EL4 mouse lymphoblastic lymphoma cell line Lane 3: U937 human histiocytic lymphoma cell line.
Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (B2M-01) - BSA Free [NB500-317]

Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (B2M-01) - BSA Free [NB500-317]

Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Confocal microscopy of human beta2-microglobulin-transfected canine cells labeled A) with B2M-01 antibody Alexa Fluor (R) 647 conjugate and B) with Alexa Fluor (R) 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2ug/ml.
Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) - BSA Free [NB500-317]

Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) - BSA Free [NB500-317]

Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Staining of beta-2-microglobulin in human peripheral blood with anti-beta-2-microglobulin (B2M-01) biotin.
View 4 more images

Applications for beta 2-Microglobulin Antibody (B2M-01) - BSA Free

Application
Recommended Usage

ELISA

1:2000

Flow Cytometry

0.5-4 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

10 ug/ml

Immunoprecipitation

1:50

Radioimmunoassay

1:100-1:2000

Western Blot

2-4 ug/ml
Application Notes
WB: positive control: U937 human histiocytic lymphoma cell line, RAJI human Burkitt lymphoma cell line, negative control: EL4 mouse lymphoblastic lymphoma cell line; both reducing and non-reducing conditions. IHC-P: positive tissue: kidney glomeruli. Flow cytometry: positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes. RIA: Use at an assay dependent concentration. The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA. IP: The antibody B2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from the lysates of human lymphoid cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using NB500-317 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: beta 2-Microglobulin

beta 2 Microglobulin non covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.

Alternate Names

B2M, beta 2Microglobulin

Gene Symbol

B2M

Additional beta 2-Microglobulin Products

Product Documents for beta 2-Microglobulin Antibody (B2M-01) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for beta 2-Microglobulin Antibody (B2M-01) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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