beta 2-Microglobulin Antibody (B2M-01) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44521
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Bovine (Negative), Canine (Negative), Chicken (Negative), Mouse (Negative), Rabbit (Negative)
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Biotin
Antibody Source
Monoclonal Mouse IgG2A Clone # B2M-01
Concentration
1 mg/ml
Product Summary for beta 2-Microglobulin Antibody (B2M-01) [Biotin]
Immunogen
Purified human beta 2 Microglobulin (NM_004048.2).
Reactivity Notes
Weak positive staining was observed in peripheral blood lymphocytes of the monkey Cercopithecus aethiops.
Specificity
The antibody B2M-01 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for beta 2-Microglobulin Antibody (B2M-01) [Biotin]
Western Blot: beta 2-Microglobulin Antibody (B2M-01) [Biotin] [NBP1-44521] - Analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human beta-t2-microglobulin (B2M-01). Lane 1: RAJI human Burkitt lymphoma cell line Lane 2: EL4 mouse lymphoblastic lymphoma cell line Lane 3: U937 human histiocytic lymphoma cell line
Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (B2M-01) [Biotin] [NBP1-44521] - Human beta-2-microglobulin-transfected canine cells labeled A) with B2M-01 antibody Alexa Fluor (R) 647 conjugate and B) with Alexa Fluor (R) 647 conjugated Mouse IgG2a Isotype Control . Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2ug/ml.
Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [Biotin] [NBP1-44521] - Staining of beta-2-microglobulin in human peripheral blood with anti-beta-2-microglobulin (B2M-01) biotin.
Applications for beta 2-Microglobulin Antibody (B2M-01) [Biotin]
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
1-2 ug/ml
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Preservative
0.9% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: beta 2-Microglobulin
Alternate Names
B2M, beta 2Microglobulin
Gene Symbol
B2M
Additional beta 2-Microglobulin Products
Product Documents for beta 2-Microglobulin Antibody (B2M-01) [Biotin]
Product Specific Notices for beta 2-Microglobulin Antibody (B2M-01) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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