beta-Catenin Antibody (15B8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53358

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Chicken

Cited:

Avian - Chicken

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 15B8

Concentration

0.2 mg/ml

View all beta-Catenin Antibodies »

Product Specifications

Immunogen

Recombinant chicken beta-Catenin (Uniprot: P35222)

Localization

Cell surface and Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54544)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for beta-Catenin Antibody (15B8)

Western Blot: beta-Catenin Antibody (15B8) [NBP2-53358]

Western Blot: beta-Catenin Antibody (15B8) [NBP2-53358]

Western Blot: beta-Catenin Antibody (15B8) [NBP2-53358] - Western Blot Analysis of HeLa cell lysate using beta-Catenin Antibody (15B8).
Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (15B8) [NBP2-53358]

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (15B8) [NBP2-53358]

Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (15B8) [NBP2-53358] - Confocal immunofluorescence image of HeLa cells using beta-Catenin Antibody (15B8) labeled is Green (CF488) and Red Dot is used to label the nuclei Red.
Immunohistochemistry-Paraffin: beta-Catenin Antibody (15B8) [NBP2-53358]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (15B8) [NBP2-53358]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (15B8) [NBP2-53358] - Formalin-fixed, paraffin-embedded human Tonsil stained with Beta-Catenin (p120) Monoclonal Antibody (15B8).

Applications for beta-Catenin Antibody (15B8)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: beta-Catenin

The protein encoded by the beta Catenin gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs arenecessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion betweencells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contactinhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this proteinbinds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this geneare a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Alternate Names

bCatenin, CTNNB1

Gene Symbol

CTNNB1

UniProt

P35222

Additional beta-Catenin Products

Product Documents for beta-Catenin Antibody (15B8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for beta-Catenin Antibody (15B8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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