beta-Catenin Antibody (15B8) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54544
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Bovine, Canine, Chicken
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 15B8
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant chicken beta-Catenin (Uniprot: P35222)
Localization
Cell surface and Cytoplasmic
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53358).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for beta-Catenin Antibody (15B8) - Azide and BSA Free
Western Blot: beta-Catenin Antibody (15B8)Azide and BSA Free [NBP2-54544]
Western Blot: beta-Catenin Antibody (15B8) - Azide and BSA Free [NBP2-54544] - Western Blot Analysis of HeLa cell lysate using beta-Catenin Antibody (15B8).Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (15B8) - Azide and BSA Free [NBP2-54544]
Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (15B8) - Azide and BSA Free [NBP2-54544] - Confocal immunofluorescence image of HeLa cells using beta-Catenin Antibody (15B8) labeled is Green (CF488) and Red Dot is used to label the nuclei Red.Immunohistochemistry-Paraffin: beta-Catenin Antibody (15B8) - Azide and BSA Free [NBP2-54544]
Immunohistochemistry-Paraffin: beta-Catenin Antibody (15B8) - Azide and BSA Free [NBP2-54544] - Formalin-fixed, paraffin-embedded human Tonsil stained with Beta-Catenin (p120) Monoclonal Antibody (15B8).Applications for beta-Catenin Antibody (15B8) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells
Immunocytochemistry/ Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
1 - 2 ug/ml
Western Blot
0.5 - 1 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: beta-Catenin
Alternate Names
bCatenin, CTNNB1
Gene Symbol
CTNNB1
Additional beta-Catenin Products
Product Documents for beta-Catenin Antibody (15B8) - Azide and BSA Free
Product Specific Notices for beta-Catenin Antibody (15B8) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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