CD133 Antibody (PROM/6316)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07898
![Immunohistochemistry-Paraffin: CD133 Antibody (PROM/6316) [NBP3-07898]](https://resources.bio-techne.com/images/products/CD133-Antibody-PROM-6316-Immunohistochemistry-Paraffin-NBP3-07898-img0002.jpg "Immunohistochemistry-Paraffin: CD133 Antibody (PROM/6316) [NBP3-07898]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, SDS-Page, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # PROM/6316
Concentration
0.2 mg/ml
Product Summary for CD133 Antibody (PROM/6316)
Immunogen
Recombinant chimericCD133 protein (around aa180-380 and aa612-765). This Mab recognizes both glycosylated and non-glycosylated extracellular domains of CD133. (Uniprot: O43490)
Localization
Apical cell Membrane Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment
Marker
Cancer Stem Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08969)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for CD133 Antibody (PROM/6316)
Immunohistochemistry-Paraffin: CD133 Antibody (PROM/6316) [NBP3-07898]
Immunohistochemistry-Paraffin: CD133 Antibody (PROM/6316) [NBP3-07898] - Formalin-fixed, paraffin-embedded human papillary renal cell carcinoma stained with CD133 / Prominin Mouse Monoclonal Antibody (PROM/6316).![Immunohistochemistry: CD133 Antibody (PROM/6316) [NBP3-07898]](https://resources.bio-techne.com/images/products/CD133-Antibody-PROM-6316-Immunohistochemistry-NBP3-07898-img0001.jpg "Immunohistochemistry: CD133 Antibody (PROM/6316) [NBP3-07898]")
Immunohistochemistry: CD133 Antibody (PROM/6316) [NBP3-07898]
Immunohistochemistry: CD133 Antibody (PROM/6316) [NBP3-07898] - Formalin-fixed, paraffin-embedded human papillary renal cell carcinoma stained with CD133 / Prominin Mouse Monoclonal Antibody (PROM/6316).Applications for CD133 Antibody (PROM/6316)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD133
Additional CD133 Products
Product Documents for CD133 Antibody (PROM/6316)
Product Specific Notices for CD133 Antibody (PROM/6316)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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