CD133 Antibody (PROM/6316) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08969CL1
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG1 Clone # PROM/6316
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CD133 Antibody (PROM/6316) [CoraFluor™ 1]
Immunogen
Recombinant chimericCD133 protein (around aa180-380 and aa612-765). This Mab recognizes both glycosylated and non-glycosylated extracellular domains of CD133. (Uniprot: O43490)
Localization
Apical cell Membrane Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment
Marker
Cancer Stem Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for CD133 Antibody (PROM/6316) [CoraFluor™ 1]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: CD133
Alternate Names
AC133, CD133, PROM1, Prominin 1, PROML1
Gene Symbol
PROM1
Additional CD133 Products
Product Documents for CD133 Antibody (PROM/6316) [CoraFluor™ 1]
Product Specific Notices for CD133 Antibody (PROM/6316) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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